Development of a multiplex TaqMan real-time PCR assay for the detection of Chlamydia psittaci and Chlamydia pneumoniae in human clinical specimens

Bernard J Wolff; Shatavia S Morrison; Jonas M Winchell

doi:10.1016/j.diagmicrobio.2017.11.014

Development of a multiplex TaqMan real-time PCR assay for the detection of Chlamydia psittaci and Chlamydia pneumoniae in human clinical specimens

Diagn Microbiol Infect Dis. 2018 Mar;90(3):167-170. doi: 10.1016/j.diagmicrobio.2017.11.014. Epub 2017 Nov 27.

Authors

Bernard J Wolff¹, Shatavia S Morrison¹, Jonas M Winchell²

Affiliations

¹ Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
² Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: jwinchell@cdc.gov.

PMID: 29291900
DOI: 10.1016/j.diagmicrobio.2017.11.014

Abstract

Diagnosis of Chlamydia psittaci and Chlamydia pneumoniae infections has traditionally relied on serological assays. We developed a multiplex real-time PCR assay for detection of C. psittaci, C. pneumoniae and an internal control. Results of this assay demonstrated 100% concordance compared to results of previously tested human clinical specimens.

Keywords: Chlamydia pneumoniae; Chlamydia psittaci; Real-time PCR.

Published by Elsevier Inc.

MeSH terms

Chlamydophila Infections / diagnosis*
Chlamydophila Infections / microbiology
Chlamydophila pneumoniae / genetics*
Chlamydophila pneumoniae / isolation & purification
Chlamydophila psittaci / genetics*
Chlamydophila psittaci / isolation & purification
DNA, Bacterial / analysis
Humans
Psittacosis / diagnosis*
Psittacosis / microbiology
RNA, Ribosomal, 16S / genetics
Real-Time Polymerase Chain Reaction / methods*
Respiratory Tract Infections / diagnosis*
Respiratory Tract Infections / microbiology
Sensitivity and Specificity

Substances

DNA, Bacterial
RNA, Ribosomal, 16S