Introduction
IgBLAST was developed at NCBI to facilitate analysis of immunoglobulin V region sequences in GenBank. It uses BLAST search algorithm.
In addition to performing a regular BLAST search, IgBLAST has several additional functions:
- Reports the germline V, D and J gene matches to the query sequence.
- Annotates the immunoglobulin domains (FWR1 through FWR3).
- Matches the returned hits (for databases other than germline genes) to the closest germline V genes, making it easier to identify related sequences.
- Reveals the V(D)J junction details such as nucleotide homology between the ends of V(D)J segments and N nucleotide insertions.
D and J gene reporting is only for nucleotide sequence search and requires a stretch of five or more nucleotide identity between the query and D or J genes.
IMPORTANT: We included only human and mouse germline genes in our current Ig germline gene collections. Accuracy has not been evaluated for query sequences from other species.
Examples are available for viewing.
Last modified: Fri Aug 10 15:10:15 EDT 2007