pan pangolin [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: panprovided by FlyBase
Official Full Name: pangolinprovided by FlyBase
Primary source: FLYBASE:FBgn0085432
Locus tag: Dmel_CG34403
See related: AllianceGenome:FB:FBgn0085432
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG17964; CG32005; CG34403; d-TCF; Dm Pan; Dmel\CG34403; dTcf; dTCF; DTcf; DTCF; IA5; l(4)102ABb; l(4)13; Lef; LEF; LEF-1; LEF/TCF; LEF/TCF-1; lef1; Lef1; LEF1; LEF1/TCF; Pan; PAN; pan.dTCF; tcf; Tcf; TCF; Tcf-1; Tcf/Lef; Tcf/LEF; TCF/LEF; TCF/LEF1; TCF/Pan
Old locus tag: CG17964; CG32005; Dmel_CG17964; Dmel_CG32005
Summary: Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and transcription corepressor binding activity. Involved in several processes, including instar larval or pupal development; regulation of gene expression; and segment polarity determination. Located in nucleus. Part of beta-catenin-TCF complex. Is expressed in embryonic brain; embryonic segment; organism; procephalic segment; and visceral mesoderm. Human ortholog(s) of this gene implicated in colorectal cancer; diabetes mellitus (multiple); pancreatic cancer; and reproductive organ cancer (multiple). Orthologous to several human genes including LEF1 (lymphoid enhancer binding factor 1) and TCF7L2 (transcription factor 7 like 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 101F1-102A3; 4-0 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	4	NC_004353.4 (69326..114270)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	4	NC_004353.3 (89952..134896)

Chromosome 4 - NC_004353.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We identify the conserved transcription factors Sox box protein 15 (Sox15) and Ftz transcription factor 1 (Ftz-f1) as genes controlled by dTcf/Pan involved in tumor development. In conclusion, this study reports a role for dTcf/Pan as a repressor of normal and oncogenic growth and identifies the genes inducing tumorigenesis downstream of dTcf/Pan.
Title: dTcf/Pangolin suppresses growth and tumor formation in Drosophila.
Requirement of Pangolin for transcriptional regulation of Wingless target genes.Pan regulates Wnt/Wg-responsive enhancers.
Title: Probing the canonicity of the Wnt/Wingless signaling pathway.
The type of HMG and Helper sites is sufficient to direct activation or repression of Wnt regulated cis-regulatory modules, and protease digestion studies suggest that TCF/Pangolin adopts distinct conformations when bound to either HMG-Helper site pair
Title: Wnt-mediated repression via bipartite DNA recognition by TCF in the Drosophila hematopoietic system.
The DNA binding ability of the C-clamp domain of pangolin is functionally important for patterning the drosophila embryo.
Title: Structure-function analysis of the C-clamp of TCF/Pangolin in Wnt/ß-catenin signaling.
TCF/Pangolin (the fly TCF) and some vertebrate TCF isoforms bind DNA through two distinct domains, a High Mobility Group (HMG) domain and a C-clamp, which recognize DNA motifs known as HMG and Helper sites, respectively.
Title: Distinct DNA binding sites contribute to the TCF transcriptional switch in C. elegans and Drosophila.
Coop (corepressor of Pan) is a Pangolin-interacting protein
Title: Coop functions as a corepressor of Pangolin and antagonizes Wingless signaling.
Data show that wingless (wg) represses transcription of the dpp gene in the ventral leg disc, and that this repression requires a tri-partite complex of the WG mediators armadillo and dTCF, and the co-repressor Brinker.
Title: Wingless directly represses DPP morphogen expression via an armadillo/TCF/Brinker complex.
data suggest that DNA recognition by TCF occurs through a bipartite mechanism, involving both the HMG domain and the C-clamp, which enables TCF to locate and activate Wnt response elements in the nucleus
Title: Activation of wingless targets requires bipartite recognition of DNA by TCF.
Bone morphogenetic protein pathway components can repress Wg target gene expression by influencing the binding of Arm and dTcf.
Title: Inhibition of Drosophila Wg signaling involves competition between Mad and Armadillo/beta-catenin for dTcf binding.
This study suggests that the sequence of TCF-binding sites allosterically regulates the TCF/Arm complex to effect either transcriptional activation or repression.
Title: Novel TCF-binding sites specify transcriptional repression by Wnt signalling.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

mobile_element

Loc: 100389-100643 mobile_element_type = transposon:INE-1{}4168
Loc: 100811-100859 mobile_element_type = transposon:1360{}4751
Loc: 111786-112228 mobile_element_type = transposon:INE-1{}4170
Loc: 71800-71849 mobile_element_type = transposon:INE-1{}4162
Loc: 75444-75627 mobile_element_type = transposon:INE-1{}4164
Loc: 76554-81921 mobile_element_type = transposon:I{}1483
Loc: 88897-89284 mobile_element_type = transposon:INE-1{}4166

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription corepressor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in canonical Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in canonical Wnt signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in canonical Wnt signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell elongation involved in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chitin-based larval cuticle pattern formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in digestive tract morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic pattern specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic pattern specification	ISS Inferred from Sequence or Structural Similarity more info
involved_in heart development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart development	TAS Traceable Author Statement more info	PubMed
involved_in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in mesodermal cell fate determination	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of stem cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in salivary gland morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in segment polarity determination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in spiracle morphogenesis, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spiracle morphogenesis, open tracheal system	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of beta-catenin-TCF complex	IBA Inferred from Biological aspect of Ancestor more info
part_of beta-catenin-TCF complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of beta-catenin-TCF complex	IPI Inferred from Physical Interaction more info	PubMed
part_of chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: pangolin

Names: CG34403-PA; CG34403-PB; CG34403-PD; CG34403-PH; CG34403-PI; CG34403-PJ; CG34403-PQ; CG34403-PR; CG34403-PS; T cell factor; T-cell factor; TCF; lethal 13; lymphocyte enhancer binding factor 1/T cell factor; pan-PA; pan-PB; pan-PD; pan-PH; pan-PI; pan-PJ; pan-PQ; pan-PR; pan-PS

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004353.4 Reference assembly

Range

69326..114270

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001258476.2 → NP_001245405.1 pangolin, isoform Q [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001245405.1

Status: REVIEWED

UniProtKB/TrEMBL

H9XVM0

Conserved Domains (2) summary

cd01388
Location:272 → 343

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...

pfam08347
Location:9 → 158

CTNNB1_binding; N-terminal CTNNB1 binding
NM_001014685.3 → NP_001014685.1 pangolin, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001014685.1

Status: REVIEWED

UniProtKB/TrEMBL

A0AVX1

Conserved Domains (2) summary

cd01388
Location:272 → 343

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...

pfam08347
Location:9 → 158

CTNNB1_binding; N-terminal CTNNB1 binding
NM_166723.3 → NP_726527.2 pangolin, isoform S [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726527.2

Status: REVIEWED

UniProtKB/TrEMBL

A0AVX1

Conserved Domains (2) summary

cd01388
Location:272 → 343

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...

pfam08347
Location:9 → 158

CTNNB1_binding; N-terminal CTNNB1 binding
NM_079880.6 → NP_524619.3 pangolin, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524619.3

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V148, B5RJS3, O02548, P91943, Q0KIE9, Q59DP5, Q5BHZ3, Q6W492, Q6W493, Q6W494, Q6W495, Q6W4A6, Q6W4B4, Q9V4B0

UniProtKB/TrEMBL

A0AVX1

Related

FBpp0088226

Conserved Domains (2) summary

cd01388
Location:272 → 343

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...

pfam08347
Location:9 → 158

CTNNB1_binding; N-terminal CTNNB1 binding
NM_001038709.3 → NP_001033798.1 pangolin, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001033798.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P91943

Conserved Domains (2) summary

cd01388
Location:272 → 343

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...

pfam08347
Location:9 → 158

CTNNB1_binding; N-terminal CTNNB1 binding
NM_001258477.2 → NP_001245406.1 pangolin, isoform R [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001245406.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V148, B5RJS3, O02548, P91943, Q0KIE9, Q59DP5, Q5BHZ3, Q6W492, Q6W493, Q6W494, Q6W495, Q6W4A6, Q6W4B4, Q9V4B0

UniProtKB/TrEMBL

A0AVX1

Conserved Domains (2) summary

cd01388
Location:272 → 343

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...

pfam08347
Location:9 → 158

CTNNB1_binding; N-terminal CTNNB1 binding
NM_166718.3 → NP_726522.1 pangolin, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726522.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V148, B5RJS3, O02548, P91943, Q0KIE9, Q59DP5, Q5BHZ3, Q6W492, Q6W493, Q6W494, Q6W495, Q6W4A6, Q6W4B4, Q9V4B0

UniProtKB/TrEMBL

A0AVX1

Related

FBpp0088228

Conserved Domains (2) summary

cd01388
Location:272 → 343

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...

pfam08347
Location:9 → 158

CTNNB1_binding; N-terminal CTNNB1 binding
NM_166720.3 → NP_726524.1 pangolin, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726524.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V148, B5RJS3, O02548, P91943, Q0KIE9, Q59DP5, Q5BHZ3, Q6W492, Q6W493, Q6W494, Q6W495, Q6W4A6, Q6W4B4, Q9V4B0

UniProtKB/TrEMBL

A0AVX1

Related

FBpp0088229

Conserved Domains (2) summary

cd01388
Location:272 → 343

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...

pfam08347
Location:9 → 158

CTNNB1_binding; N-terminal CTNNB1 binding
NM_166724.4 → NP_726528.2 pangolin, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726528.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A0AVW4, Q8IMA8

Conserved Domains (1) summary

cd01388
Location:713 → 784

SOX-TCF_HMG-box; SOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and ...