MET4 Met4p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MET4
Gene description: Met4p
Primary source: SGD:S000005047
Locus tag: YNL103W
See related: AllianceGenome:SGD:S000005047
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables transcription coactivator activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; response to arsenic-containing substance; and response to cadmium ion. Located in nucleus. Part of Cbf1-Met4-Met28 complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (427735..429753)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Ubiquitin Interacting Motif-Like Domain of Met4 Selectively Binds K48 Polyubiquitin Chains.
Title: The Ubiquitin Interacting Motif-Like Domain of Met4 Selectively Binds K48 Polyubiquitin Chains.
Impairment of MET transcriptional activators, MET4 and MET31 induced lipid accumulation in Saccharomyces cerevisiae.
Title: Impairment of MET transcriptional activators, MET4 and MET31 induced lipid accumulation in Saccharomyces cerevisiae.
Met4, Ino2, and Gcn4 have been shown to have similar functions in transcription activation assays and to require Med15 for activation of Mediator tail-dependent promoters, and both ADs bind Med15 activator-binding domains with low micromolar affinities.
Title: Transcription Activation Domains of the Yeast Factors Met4 and Ino2: Tandem Activation Domains with Properties Similar to the Yeast Gcn4 Activator.
We provided evidence that these three patterns of MET3pr expression are caused by Sir2-mediated silencing, transcriptional interference, and 3D clustering. The clustering also occurs in the native genome and enhances the transcription of endogenous Met4-targeted genes.
Title: Three distinct mechanisms of long-distance modulation of gene expression in yeast.
In Saccharomyces cerevisiae, transcription of the MET regulon is controlled by ubiquitination of the transcription factor Met4, which is carried out by the SCF(Met30) E3 ubiquitin ligase.
Title: Multiple inputs control sulfur-containing amino acid synthesis in Saccharomyces cerevisiae.
Data suggest that Met4 cofactors use a variety of mechanisms to allow differential transcription of target genes in response to various cues.
Title: Dissection of combinatorial control by the Met4 transcriptional complex.
Met32 has a regulatory role as part of the Cbf1-Met4 complex as shown by the met30 cell cycle defect
Title: A dominant suppressor mutation of the met30 cell cycle defect suggests regulation of the Saccharomyces cerevisiae Met4-Cbf1 transcription complex by Met32.
The overexpression of Cdc34 accelerated the ubiquitination of the transcription factor Met4 and reduced expression of MET25 gene, which is a target of Met4.
Title: Ubiquitin-conjugating enzyme Cdc34 mediates cadmium resistance in budding yeast through ubiquitination of the transcription factor Met4.
the recruitment of Met4p to the SCF(Met30p) complex itself results in inactivation of Met4p, independently of its ubiquitination
Title: Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p.
Identification of a ubiquitin-binding domains (UBD) in the yeast transcription factor Met4.
Title: A ubiquitin-interacting motif protects polyubiquitinated Met4 from degradation by the 26S proteasome.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cysteine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in methionine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to arsenic-containing substance	IDA Inferred from Direct Assay more info	PubMed
involved_in response to cadmium ion	IDA Inferred from Direct Assay more info	PubMed
involved_in sulfur amino acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cbf1-Met4-Met28 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cbf1-Met4-Met28 complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: Met4p

NP_014296.4

Leucine-zipper transcriptional activator; responsible for regulation of sulfur amino acid pathway; requires different combinations of auxiliary factors Cbf1p, Met28p, Met31p and Met32p; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; can be ubiquitinated by ubiquitin ligase SCF-Met30p, is either degraded or maintained in an inactive state; regulates degradation of its own DNA-binding cofactors by targeting them to SCF-Met30p