PAB1 polyadenylate-binding protein [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PAB1
Gene description: polyadenylate-binding protein
Primary source: SGD:S000000967
Locus tag: YER165W
See related: AllianceGenome:SGD:S000000967
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables several functions, including poly(A) binding activity; promoter-specific chromatin binding activity; and protein kinase A catalytic subunit binding activity. Involved in regulation of nuclear-transcribed mRNA poly(A) tail shortening and regulation of translational initiation. Acts upstream of or within mRNA 3'-end processing. Located in cytoplasmic stress granule; nucleus; and ribosome. Orthologous to several human genes including PABPC1 (poly(A) binding protein cytoplasmic 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (510373..512106)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Poly(A)-Binding Protein Regulates the Efficiency of Translation Termination.
Title: Poly(A)-Binding Protein Regulates the Efficiency of Translation Termination.
Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.
Title: Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.
The level of Pab1 binding to poly(A)-containing isoforms is surprisingly variable, and differences in Pab1 binding correlate with the extent of structural variation for closely spaced isoforms.
Title: Extensive Structural Differences of Closely Related 3' mRNA Isoforms: Links to Pab1 Binding and mRNA Stability.
The authors found that Pab1 association into these aggregates relies mainly on RNA recognition motifs, whose number is important for an efficient recruitment of the protein.
Title: The recruitment of the Saccharomyces cerevisiae poly(A)-binding protein into stress granules: new insights into the contribution of the different protein domains.
Two distal RNA recognition motif of Sbp1 bind to the poly(A) sequence in the 5'UTR of the Pab1 mRNA specifically and cooperatively while the central RGG domain of the protein interacts directly with Pab1.
Title: Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner.
Study shows that Pab1 phase separates and gels in response to physiological thermal and pH stresses, a demixing process, which is modulated by the P domain but does not require it; Poly(A)-binding protein thus acts as a physiological stress sensor, exploiting phase separation to precisely mark stress onset, a broadly generalizable mechanism.
Title: Stress-Triggered Phase Separation Is an Adaptive, Evolutionarily Tuned Response.
Closed-loop model of mRNA translation is prevalent for eIF4E/4G-bound but unexpectedly sparse for PAB1-bound mRNAs.
Title: Probing the closed-loop model of mRNA translation in living cells.
In Saccharomyces cerevisiae the association of Pab1 with eRF3 negatively regulates translation termination.
Title: Interaction between the poly(A)-binding protein Pab1 and the eukaryotic release factor eRF3 regulates translation termination but not mRNA decay in Saccharomyces cerevisiae.
measured the contribution of each structural element in RRM2 domain to Pab1 performance, dissected the in vivo effects of mutations at known RNA-binding residues and other interaction sites, and identified non-RNA-binding residues essential to RRM2 function
Title: Deep mutational scanning of an RRM domain of the Saccharomyces cerevisiae poly(A)-binding protein.
These results suggest that the RRM1 domain is the more critical region of PAB1 for controlling deadenylation and consists of at least two distinguishable functional regions.
Title: The RRM1 domain of the poly(A)-binding protein from Saccharomyces cerevisiae is critical to control of mRNA deadenylation.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables poly(A) binding	IBA Inferred from Biological aspect of Ancestor more info
enables poly(U) RNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within mRNA 3'-end processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasmic stress granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ribonucleoprotein complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: polyadenylate-binding protein

NP_011092.1

Poly(A) binding protein; interacts with the cleavage factor complex CF I, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex; role in preventing L-A mycovirus pathogenesis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

510373..512106

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179055.1 → NP_011092.1 TPA: polyadenylate-binding protein [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011092.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM73, P04147

UniProtKB/TrEMBL

A0A6A5Q0F2, A6ZRC1, B5VHU1, C7GS45, C8Z7I7, N1P5F4

Conserved Domains (1) summary

TIGR01628
Location:38 → 559

PABP-1234; polyadenylate binding protein, human types 1, 2, 3, 4 family