BMH1 14-3-3 family protein BMH1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: BMH1
Gene description: 14-3-3 family protein BMH1
Primary source: SGD:S000000979
Locus tag: YER177W
See related: AllianceGenome:SGD:S000000979
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: APR6
Summary: Enables DNA replication origin binding activity; RNA polymerase II-specific DNA-binding transcription factor binding activity; and phosphoserine residue binding activity. Involved in several processes, including aggresome assembly; intracellular signal transduction; and negative regulation of nitrogen compound metabolic process. Located in cytoplasm and nucleus. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 56; transitional cell carcinoma; and vulva squamous cell carcinoma. Orthologous to several human genes including YWHAE (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (545611..546414)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

14-3-3 Protein Bmh1 triggers short-range compaction of mitotic chromosomes by recruiting sirtuin deacetylase Hst2.
Title: 14-3-3 Protein Bmh1 triggers short-range compaction of mitotic chromosomes by recruiting sirtuin deacetylase Hst2.
Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84
Title: Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84.
Both Bmh1 and Bmh2 associate with the promoters of at least some of these genes. BMH2, but not BMH1, attenuates the repression of genes involved in some functions required for ribosome biogenesis. BMH2 also attenuates the activation of genes sensitive to nitrogen catabolite repression.
Title: The yeast 14-3-3 proteins BMH1 and BMH2 differentially regulate rapamycin-mediated transcription.
Here we show that the defect in glucose-repression in the absence of Ssb is due to the ability of the chaperone to bridge between the SNF1 and Glc7 complexes. Ssb performs this post-translational function in concert with the 14-3-3 protein Bmh, to which Ssb binds via its very C-terminus. Raising the intracellular concentration of Ssb or Bmh enabled Glc7 to dephosphorylate SNF1 even in the absence of Reg1
Title: The Hsp70 homolog Ssb and the 14-3-3 protein Bmh1 jointly regulate transcription of glucose repressed genes in Saccharomyces cerevisiae.
Bmh1 is required for nuclear Gln3 localization and appears to function downstream of Ure2.
Title: General Amino Acid Control and 14-3-3 Proteins Bmh1/2 Are Required for Nitrogen Catabolite Repression-Sensitive Regulation of Gln3 and Gat1 Localization.
Data indicate that transcription activation is regulated negatively by two redundant 14-3-3 isoforms, Bmh1 and Bmh2.
Title: Yeast 14-3-3 protein functions as a comodulator of transcription by inhibiting coactivator functions.
Bmh1 is a spindle position checkpoint (SPOC) component that breaks Bfa1 centrosome asymmetry upon SPOC activation.
Title: The 14-3-3 protein Bmh1 functions in the spindle position checkpoint by breaking Bfa1 asymmetry at yeast centrosomes.
EF-hand like motif-containing domain functions as the intermediary through which the 14-3-3 protein modulates the function of the catalytic domain of Nth1
Title: Role of the EF-hand-like motif in the 14-3-3 protein-mediated activation of yeast neutral trehalase Nth1.
Bmh1 and Bmh2 inhibit transcription by binding to Adr1 at promoters that contain a preinitiation complex, demonstrating that Bmh-mediated inhibition is not due to nuclear exclusion, inhibition of DNA binding, or RNA polymerase II (Pol II) recruitment.
Title: 14-3-3 (Bmh) proteins regulate combinatorial transcription following RNA polymerase II recruitment by binding at Adr1-dependent promoters in Saccharomyces cerevisiae.
Data show that Bmh binds to the Snf1-dependent transcription factor Adr1 and inhibits its transcriptional activity.
Title: 14-3-3 (Bmh) proteins inhibit transcription activation by Adr1 through direct binding to its regulatory domain.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphoserine residue binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Ras protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in aggresome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ascospore formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fungal-type cell wall chitin biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle orientation checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein ubiquitination	IPI Inferred from Physical Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in pseudohyphal growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of glycogen metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in signal transduction involved in filamentous growth	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: 14-3-3 family protein BMH1

NP_011104.3

14-3-3 protein, major isoform; involved in post-transcriptional control of the proteome; binds to both proteins and to DNA, including replication origins; regulates exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, the spindle position checkpoint and meiotic commitment; protein abundance and relative distribution to the nucleus increase upon DNA replication stress; antiapoptotic gene; similar to several human 14-3-3 proteins

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

545611..546414

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179067.3 → NP_011104.3 TPA: 14-3-3 family protein BMH1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011104.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM86, P29311, Q06854

UniProtKB/TrEMBL

A0A6A5PXL2, A6ZRD4, B3LRT7, B5VHV9, C7GS32, C8Z7K1, N1P3Q2

Conserved Domains (1) summary

COG5040
Location:1 → 267

BMH1; 14-3-3 family protein [Signal transduction mechanisms]