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    HTB1 histone H2B [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851810, updated on 13-Apr-2024

    Summary

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    Gene symbol
    HTB1
    Gene description
    histone H2B
    Primary source
    SGD:S000002632
    Locus tag
    YDR224C
    See related
    AllianceGenome:SGD:S000002632
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    SPT12
    Summary
    Enables DNA binding activity. Involved in chromatin assembly or disassembly; negative regulation of transcription by RNA polymerase II; and postreplication repair. Part of nucleosome and replication fork protection complex. Orthologous to several human genes including H2BC11 (H2B clustered histone 11); H2BC12 (H2B clustered histone 12); and H2BC13 (H2B clustered histone 13). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (914317..914712, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Crf1p Neighboring gene histone H2A Neighboring gene adenylate kinase ADK1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Reversal of histone H2B mono-ubiquitination is required for replication stress recovery. Korenfeld HT, et al. DNA Repair (Amst), 2022 Nov. PMID 36058129
    2. A Mutation in Histone H2B Represents a New Class of Oncogenic Driver. Bennett RL, et al. Cancer Discov, 2019 Oct. PMID 31337617, Free PMC Article
    3. Deciphering the roles of the histone H2B N-terminal domain in genome-wide transcription. Parra MA, et al. Mol Cell Biol, 2006 May. PMID 16648479, Free PMC Article
    4. A sequence element downstream of the yeast HTB1 gene contributes to mRNA 3' processing and cell cycle regulation. Campbell SG, et al. Mol Cell Biol, 2002 Dec. PMID 12446762, Free PMC Article
    5. Chromatin assembly in a yeast whole-cell extract. Schultz MC, et al. Proc Natl Acad Sci U S A, 1997 Aug 19. PMID 9256430, Free PMC Article

    See all (340) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Reversal of histone H2B mono-ubiquitination is required for replication stress recovery.
      Title: Reversal of histone H2B mono-ubiquitination is required for replication stress recovery.
    2. Influence of sequence length and charged residues on Swc5 binding with histone H2A-H2B.
      Title: Influence of sequence length and charged residues on Swc5 binding with histone H2A-H2B.
    3. RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.
      Title: RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.
    4. Bre1 mediates the ubiquitination of histone H2B by regulating Lge1 stability
      Title: Bre1 mediates the ubiquitination of histone H2B by regulating Lge1 stability.
    5. This study not only demonstrates the applications of the versatile histone library, but also reveals many previously unknown functions of histone H2A and H2B.
      Title: Dissecting Nucleosome Function with a Comprehensive Histone H2A and H2B Mutant Library.
    6. Data indicate that histone H2B ubiquitylation (H2Bub) and Asf1 cooperatively promote transcriptional silencing at telomeres and mating loci.
      Title: H2B ubiquitylation and the histone chaperone Asf1 cooperatively mediate the formation and maintenance of heterochromatin silencing.
    7. A Nap1 dimer provides an acidic binding surface and asymmetrically engages a single H2A-H2B heterodimer.
      Title: Structural evidence for Nap1-dependent H2A-H2B deposition and nucleosome assembly.
    8. Chd1 takes part in chromatin reorganization surrounding transcribing RNA polymerase II (Pol II) in asymmetric nucleosomes; ubiquitin-conjugated histone H2B stimulates nucleosome sliding by Chd1.
      Title: The Chd1 chromatin remodeler shifts hexasomes unidirectionally.
    9. decreased CKII function in vivo results in decreased levels of histone H2B lysine 123 monoubiquitylation, a modification dependent on FACT and PAF-C.
      Title: Quantitative Analysis of Dynamic Protein Interactions during Transcription Reveals a Role for Casein Kinase II in Polymerase-associated Factor (PAF) Complex Phosphorylation and Regulation of Histone H2B Monoubiquitylation.
    10. Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo.
      Title: Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus.
    Submit: New GeneRIF Correction See all GeneRIFs (17)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein heterodimerization activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables structural constituent of chromatin IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in chromatin organization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in postreplication repair IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of nucleosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of nucleosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of protein-containing complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of replication fork protection complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    histone H2B
    NP_010510.3
    • Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      914317..914712 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180532.3 → NP_010510.3  TPA: histone H2B [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010510.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VSK6, P02293
      UniProtKB/TrEMBL
      A0A6A5PZQ7, A6ZYH8, B3LG63, C7GVC8, C8Z5D5, G2WAW9, N1P503
      Conserved Domains (1) summary
      smart00427
      Location:31 → 127
      H2B; Histone H2B
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    4KUD_D.1
    4KUD_H.1
    6GEJ_G.1
    6GEJ_H.1
    6GEN_G.1
    6GEN_H.1
    7K78_D.1
    7K78_H.1
    7K7G_D.1
    7K7G_H.1
    P02293.2

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