U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    fnr DNA-binding transcriptional dual regulator FNR [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945908, updated on 4-Feb-2024

    Summary

    Go to the top of the page Help
    Gene symbol
    fnr
    Gene description
    DNA-binding transcriptional dual regulator FNR
    Primary source
    ASAP:ABE-0004475
    Locus tag
    b1334
    See related
    ECOCYC:EG10325
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1330; nirA; nirR; ossA; oxrA
    Summary
    FNR represses genes involved in aerobic respiration and activates genes required for anaerobic respiration. [More information is available at EcoGene: EG10325]. FNR is a dual DNA-binding transcriptional regulator which stimulates the transcription of many genes required for fermentation and anaerobic respiration. [More information is available at EcoCyc: EG10325].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (1398774..1399526, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2534 domain-containing protein YnaJ Neighboring gene universal stress protein E Neighboring gene methylated-DNA--[protein]-cysteine S-methyltransferase Neighboring gene p-aminobenzoyl glutamate:H(+) symporter

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. A variant of the Escherichia coli anaerobic transcription factor FNR exhibiting diminished promoter activation function enhances ionizing radiation resistance. Bruckbauer ST, et al. PLoS One, 2019. PMID 30673695, Free PMC Article
    2. FNR regulates expression of important virulence factors contributing to pathogenicity of uropathogenic Escherichia coli. Barbieri NL, et al. Infect Immun, 2014 Dec. PMID 25245807, Free PMC Article
    3. Influence of association state and DNA binding on the O₂-reactivity of [4Fe-4S] fumarate and nitrate reduction (FNR) regulator. Crack JC, et al. Biochem J, 2014 Oct 1. PMID 25019503, Free PMC Article
    4. Genome-scale analysis of escherichia coli FNR reveals complex features of transcription factor binding. Myers KS, et al. PLoS Genet, 2013 Jun. PMID 23818864, Free PMC Article
    5. Covalently linking the Escherichia coli global anaerobic regulator FNR in tandem allows it to function as an oxygen stable dimer. Shan Y, et al. Biochem Biophys Res Commun, 2012 Mar 2. PMID 22326866

    See all (160) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The FNR F186I variant is apparently capable of enhancing resistance to ionizing radiation under chronic irradiation conditions, but does not increase cell survival when exposed to acute irradiation.
      Title: A variant of the Escherichia coli anaerobic transcription factor FNR exhibiting diminished promoter activation function enhances ionizing radiation resistance.
    2. Elevated expression of GlpT and UhpT via FNR activation contributes to increased fosfomycin susceptibility in Escherichia coli under anaerobic conditions.
      Title: Elevated Expression of GlpT and UhpT via FNR Activation Contributes to Increased Fosfomycin Susceptibility in Escherichia coli under Anaerobic Conditions.
    3. These results demonstrate that FNR is a key global regulator of uropathogenic Escherichia coli virulence and controls expression of important virulence factors that contribute to uropathogenic Escherichia coli pathogenicity.
      Title: FNR regulates expression of important virulence factors contributing to pathogenicity of uropathogenic Escherichia coli.
    4. DNA-bound FNR reacts more rapidly with O2 than FNR free in solution, implying that transcriptionally active FNR is the preferred target for reaction with O2.
      Title: Influence of association state and DNA binding on the O₂-reactivity of [4Fe-4S] fumarate and nitrate reduction (FNR) regulator.
    5. FNR bound some promoters without regulating expression presumably requiring changes in activity of condition-specific transcription factors. Such combinatorial regulation may allow Escherichia coli to respond rapidly to environmental changes
      Title: Genome-scale analysis of escherichia coli FNR reveals complex features of transcription factor binding.
    6. oxygen stable FNR variants were obtained.
      Title: Covalently linking the Escherichia coli global anaerobic regulator FNR in tandem allows it to function as an oxygen stable dimer.
    7. The authors characterized the steady-state behavior of the FNR system by determining its sensitivity to changes in parameter values and its response to changes in the concentration of iron-sulfur cluster assembly proteins and the protease ClpXP.
      Title: Regulation of aerobic-to-anaerobic transitions by the FNR cycle in Escherichia coli.
    8. The authors show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element.
      Title: Activation and repression at the Escherichia coli ynfEFGHI operon promoter.
    9. Results suggest that the Fnr and Crp proteins may act synergistically to enhance NapABC synthesis during growth with poor carbon sources to help obtain energy from low levels of nitrate.
      Title: Catabolite repression control of napF (periplasmic nitrate reductase) operon expression in Escherichia coli K-12.
    10. transcriptomic data identified the global transcription regulators ArcA, CRP, Fis, FNR, Fur, BaeR, CpxR, and IHF as targets and potential CO sensors
      Title: Carbon monoxide-releasing antibacterial molecules target respiration and global transcriptional regulators.
    Submit: New GeneRIF Correction See all GeneRIFs (21)

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription activator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables DNA-binding transcription activator activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-binding transcription factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables iron-sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transcription cis-regulatory region binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within anaerobic respiration IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of DNA-templated transcription IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to nitric oxide IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-DNA complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    part_of protein-DNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    DNA-binding transcriptional dual regulator FNR

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1398774..1399526 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415850.1 DNA-binding transcriptional dual regulator FNR [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415850.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X3K777
      Conserved Domains (1) summary
      PRK11161
      Location:11 → 245
      PRK11161; fumarate/nitrate reduction transcriptional regulator Fnr
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.