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    galK galactokinase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945358, updated on 11-Apr-2024

    Summary

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    Gene symbol
    galK
    Gene description
    galactokinase
    Primary source
    ASAP:ABE-0002568
    Locus tag
    b0757
    See related
    ECOCYC:EG10363
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0746; galA
    Summary
    An amber mutation has been generated . [More information is available at EcoCyc: EG10363].
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    Genomic context

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    Sequence:
    NC_000913.3 (788831..789979, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-deoxy-7-phosphoheptulonate synthase, Phe-sensitive Neighboring gene 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase Neighboring gene galactose-1-epimerase Neighboring gene galactose-1-phosphate uridylyltransferase Neighboring gene UDP-glucose 4-epimerase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene. Wang X, et al. Proc Natl Acad Sci U S A, 2015 Jun 16. PMID 26045496, Free PMC Article
    2. Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization. Yang J, et al. Chem Biol, 2005 Jun. PMID 15975511
    3. Simple and highly efficient BAC recombineering using galK selection. Warming S, et al. Nucleic Acids Res, 2005 Feb 24. PMID 15731329, Free PMC Article
    4. Mechanistic implications of Escherichia coli galactokinase structure-based engineering. Hoffmeister D, et al. Chembiochem, 2004 Jul 5. PMID 15239057
    5. Creation of the first anomeric D/L-sugar kinase by means of directed evolution. Hoffmeister D, et al. Proc Natl Acad Sci U S A, 2003 Nov 11. PMID 14612558, Free PMC Article

    See all (41) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Single-mutations at the galactose-binding site of enzymes GalK, GalU, and LgtC enable the efficient synthesis of UDP-6-azido-6-deoxy-d-galactose and azido-functionalized Gb3 analogs.
      Title: Single-mutations at the galactose-binding site of enzymes GalK, GalU, and LgtC enable the efficient synthesis of UDP-6-azido-6-deoxy-d-galactose and azido-functionalized Gb3 analogs.
    2. type 2 polarity occurs as a result of the down-regulation of galK, which is caused by two mechanisms: Spot 42-mediated degradation of the galK-specific mRNA, mK2, and Spot 42-mediated Rho-dependent transcription termination at the end of galT
      Title: Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae.
    4. 6-azido-6-deoxy-galactose and 6-azido-6-deoxy-glucose present unique chemical probes to assess the utility of an E. coli Y371H/M173L-GalK-overproducing strain to generate unnatural sugar-1-phosphates in vivo
      Title: Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables galactokinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables galactokinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables galactokinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables galactokinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphotransferase activity, alcohol group as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in carbohydrate phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within galactose catabolic process via UDP-galactose IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in galactose metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in galactose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    galactokinase
    NP_415278.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      788831..789979 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415278.1 galactokinase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415278.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T6PVM9
      Conserved Domains (1) summary
      PRK05101
      Location:1 → 382
      PRK05101; galactokinase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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