dna2.S DNA replication helicase/nuclease 2 S homeolog [Xenopus laevis (African clawed frog)] - Gene

Summary

Official Symbol: dna2.S
Official Full Name: DNA replication helicase/nuclease 2 S homeolog
Primary source: Xenbase:XB-GENE-5829000
Locus tag: XELAEV_18037088mg
See related: EnsemblRapid:ENSXLAG00005035492
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Xenopus laevis
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus
Also known as: dna2; XDna2; dna2l; dna2-A
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: chromosome: 7S

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
101	current	Xenopus_laevis_v10.1 (GCF_017654675.1)	7S	NC_054384.1 (29415221..29458864)
100	previous assembly	Xenopus_laevis_v2 (GCF_001663975.1)	7S	NC_030737.1 (27900689..27944536)

Chromosome 7S - NC_054384.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that cleavage of free 5' DNA ends at clean double-strand breaks (DSBs) is mediated by DNA replication helicase/nuclease 2 (Dna2).
Title: Dna2 initiates resection at clean DNA double-strand breaks.
Dna2 co-localizes in foci with RPA and is found in a complex with replication fork components And-1 and Mcm10. Dna2 interacts with the DSB repair and checkpoint proteins Nbs1 and ATM.
Title: Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM.
Results provide strong evidence that xDNA2 is a major nuclease for the resection of DNA ends for homology-dependent DNA double-strand break repair in eukaryotes.
Title: Identification of the Xenopus DNA2 protein as a major nuclease for the 5'->3' strand-specific processing of DNA ends.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Gene Ontology Provided by Xenbase

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables 5'-3' DNA helicase activity	ISS Inferred from Sequence or Structural Similarity more info
enables 5'-flap endonuclease activity	IEA Inferred from Electronic Annotation more info
enables 5'-flap endonuclease activity	ISS Inferred from Sequence or Structural Similarity more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	ISS Inferred from Sequence or Structural Similarity more info
NOT enables DNA helicase activity	ISS Inferred from Sequence or Structural Similarity more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	ISS Inferred from Sequence or Structural Similarity more info
enables single-stranded DNA helicase activity	ISS Inferred from Sequence or Structural Similarity more info
enables site-specific endodeoxyribonuclease activity, specific for altered base	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA double-strand break processing	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA replication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA replication	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA replication checkpoint signaling	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA replication, Okazaki fragment processing	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA replication, removal of RNA primer	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within base-excision repair	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within double-strand break repair via single-strand annealing	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within mitochondrial DNA replication	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of DNA replication	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within replication fork reversal	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of gamma DNA polymerase complex	ISS Inferred from Sequence or Structural Similarity more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: DNA replication ATP-dependent helicase/nuclease DNA2

Names: DNA replication ATP-dependent helicase-like homolog; DNA replication helicase 2 homolog; DNA2-like helicase; helicase

NP_001079231.1

EC 3.6.4.12

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001085762.1 → NP_001079231.1 DNA replication ATP-dependent helicase/nuclease DNA2

See identical proteins and their annotated locations for NP_001079231.1

Status: PROVISIONAL

Source sequence(s)

AF474372

UniProtKB/Swiss-Prot

Q8QHA5

UniProtKB/TrEMBL

A0A974CC12

Related

ENSXLAP00005106127.1, ENSXLAT00005108203.1

Conserved Domains (5) summary

TIGR00376
Location:469 → 1031

TIGR00376; DNA helicase, putative

pfam08696
Location:76 → 283

Dna2; DNA replication factor Dna2

pfam13087
Location:806 → 1018

AAA_12; AAA domain

cl00641
Location:262 → 358

Cas4_I-A_I-B_I-C_I-D_II-B; CRISPR/Cas system-associated protein Cas4

cl21455
Location:626 → 850

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases