|
| Status |
Public on Jul 22, 2013 |
| Title |
African American [GM17140] |
| Sample type |
genomic |
| |
|
| Source name |
GM17140
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: GM17140
gender: Female
health state: Healthy donor
|
| Biomaterial provider |
Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM17140
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was quantified by Quant-iT PicoGreen dsDNA Reagent (Invitrogen) and the integrity was analyzed in a 1.3% agarose gel.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
| Description |
healthy sample
|
| Data processing |
The intensities of the images were extracted using GenomeStudio (V2011.2) Methylation module (1.8.5) software. The methylation score of each CpG is represented as a beta value.
|
| |
|
| Submission date |
Apr 27, 2012 |
| Last update date |
Jul 22, 2013 |
| Contact name |
Manel Esteller |
| Organization name |
IDIBELL
|
| Department |
PEBC
|
| Lab |
Cancer Epigenetics
|
| Street address |
Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
|
| City |
L'Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE36369 |
DNA methylation contributes to natural human variation |
|
