|
| Status |
Public on Mar 20, 2012 |
| Title |
Genotype_777_0dpa_fiber_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Fiber 0day post anthesis
|
| Organism |
Gossypium hirsutum |
| Characteristics |
genotype: JKC 777
development stage: 0 dpa fiber
|
| Treatment protocol |
During the flowering season flowers were tagged on anthesis and harvested at six different developmental time points: 0, 6, 9, 12, 19 & 25 days post anthesis (dpa). For RNA isolation whole ovule was taken as 0dpa sample whereas for rest of the stages only fibers were the starting material.
|
| Growth protocol |
All five Genotype of G. hirsutum were sown in the open field at National Botanical Research Institute, Lucknow, India and grown in natural condition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from Spectrum plant total RNA Kit (Sigma-Aldrich) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip CottonGenome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Gene chip scanner 3000.
|
| Description |
777 superior traits of fiber quality
Gene expression data from 0DPA fiber from Gossypium hirsutum
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Mar 02, 2012 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Samir V Sawant |
| E-mail(s) |
samirsawant@nbri.res.in
|
| Phone |
+91-522-2297947
|
| Fax |
+91-0522-2205839
|
| URL |
http://www.nbri-lko.org/
|
| Organization name |
National Botanical Research Institute
|
| Department |
Centre for Plant and Molecular Breeding
|
| Lab |
Plant Molecular Biology
|
| Street address |
Sikandrabad
|
| City |
Lucknow |
| ZIP/Postal code |
226001 |
| Country |
India |
| |
|
| Platform ID |
GPL8672 |
| Series (1) |
| GSE36228 |
Affymetrix Cotton Genome array expression data of cotton fiber at different developmental stages from different varieties of Gossypium hirsutum |
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