|
| Status |
Public on Jan 26, 2012 |
| Title |
313T_MRL/lpr positive control |
| Sample type |
protein |
| |
|
| Source name |
murine antiserum
|
| Organism |
Mus musculus |
| Characteristics |
strain: MRL/lpr
treatment: positive control
|
| Extracted molecule |
protein |
| Extraction protocol |
Whole antiserum was obtained.
|
| Label |
DyLight-649-conjugated goat anti-mouse IgM antibody
|
| Label protocol |
Commercially available DyLight 649-conjugated goat anti-mouse antibody was added to the microarray at 1:2000 in 5% fetal bovine serum in phosphate-buffered saline with 0.1% Tween-20.
|
| |
|
| Hybridization protocol |
Murine antiserum was probed on the antigen microarray at 1:250 dilution in 5% fetal bovine serum in phosphate-buffered saline with 0.1% Tween-20, and incubated for 1.25 hours at 4 degrees Celsius.
|
| Scan protocol |
Fluorescent array images were collected at 635 nm with a GenePix 4000B fluorescent scanner at 10 micron resolution and image intensity data were extracted and analyzed with GenePix Pro 6.1 analysis software.
|
| Description |
313T
Murine antiserum was obtained from blood collected from an individual MLR/lpr mouse exhibiting lupus-like symptoms. A 1:250 dilution of this antiserum was then applied onto the Utz Lab Whole Protein Autoantigen Array (V2.0), labeled with a goat anti-mouse IgM secondary antibody conjugated to DyLight-649 (Jackson ImmunoResearch), and scanned with a GenePix 4000B scanner using GenePix Pro 6.1.
|
| Data processing |
Data were background corrected and flagged values were removed. The remaining replicate values were averaged.
|
| |
|
| Submission date |
Oct 01, 2011 |
| Last update date |
Jan 26, 2012 |
| Contact name |
Chih Long Liu |
| Organization name |
Stanford University
|
| Department |
Medicine - Imm. & Rheum
|
| Lab |
Utz
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL14638 |
| Series (2) |
| GSE32542 |
Murine serum reactivity to common autoantigens in response to immunization with neutrophil extracellular traps |
| GSE32544 |
Specific post-translational histone mod. of neutrophil extracellular traps as immunogens & potential SLE Ab targets. |
|
