strain: C57BL/6 tissue: Brain age: Postnatal day seven hypoxia or normoxia group: Hypoxia reoxygenated with: 21 % O2
Extracted molecule
total RNA
Extraction protocol
Ficoll isopaque gradient centrifugation and RiboPure Blood Kit (Ambion, Life Technologies, Carlsbad, USA) were used for total RNA extraction
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol
GeneChip Hybridization Oven, 16-18 hours, 45oC, 60 rotations/min; washing and staining on Fluidics Station 450, protocol FS450_0007
Scan protocol
GeneChip Scanner 3000 7G controlled by GeneChip Operating Software
Description
H_21_Brain_4
Data processing
Robust multiarray average (R/Bioconductor, using the library aroma.affymetrix)
