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Sample GSM794477 Query DataSets for GSM794477
Status Public on Nov 23, 2011
Title Serum samples of healthy controls (HC) 1
Sample type protein
 
Source name serum
Organism Homo sapiens
Characteristics disease state: healthy HC
block in gpr file: 5
sample type: serum
Extracted molecule protein
Extraction protocol serum samples
Label Cy3
Label protocol According to manufacturer's protocols. In brief, 70ul of 1,500 fold diluted Fluorescent dye-conjugated streptavidin ws added to each subarray and incubated in a dark room at room temperature for 1-2 hours.
 
Hybridization protocol The array consisted of three glass slides (array 6, 7, and 8) that were pretreated according to the manufacturer's instructions and incubated with 2-fold diluted serum pools for 2 hours. All sample measurements were performed in duplicate. The array glass slides were washed, incubated with a biotin-conjugated anti-cytokine mix for 2 hours, washed again, and developed for 2 hours with Cy3-conjugated streptavidin.
Scan protocol Fluorescence intensities (Cy3) were recorded with an Axon GenePix 4000B scanner.
Description pool A of 10 serum samples
Data processing Raw data was analyzed with the Raybiotech analysis tool, a data analysis program based on Microsoft Excel technology specifically designed for the analysis of these microarrays. Signals were normalized using internal, positive and negative controls included on the array. Signals were normalized using positive and negative control features on the microarray, the fold changes are relative expression to positive control features, i.e. a value of 1 is same expression than positive control, 2 a 2 fold higher, 0.5 a 2-fold lower expression.
 
Submission date Sep 09, 2011
Last update date Nov 24, 2011
Contact name Johannes Rainer
E-mail(s) johannes.rainer@eurac.edu
Organization name Eurac Researc
Department Institute for Biomedicine
Lab Biomedical Informatics
Street address Via A. Volta 21
City Bolzano
ZIP/Postal code 39100
Country Italy
 
Platform ID GPL14559
Series (2)
GSE32037 Identification of potential biomarkers for patients with neurodegenerative parkinsonian syndromes using serum cytokine microarray analysis; series 6
GSE32041 Identification of potential biomarkers for patients with neurodegenerative parkinsonian syndromes using serum cytokine microarray analysis

Data table header descriptions
ID_REF
VALUE Relative expression compared to internal control features. Fold change in natural scale.

Data table
ID_REF VALUE
1 1.390565661
2 0.058914651
3 0.011692984
4 0.006895862
5 0.013941635
6 0.00089946
7 0.017689386
8 0.287827304
9 0.014991005
10 0.029682191
11 0.007495503
12 0.035528683
13 0.010943434
14 0.036278233
15 0.006296222
16 0.007945233
17 0.022636418
18 0.014991005
19 0.006146312
20 0.085148911

Total number of rows: 60

Table truncated, full table size <1 Kbytes.




Supplementary file Size Download File type/resource
GSM794477_14102620_2010-01-26-6.gpr.gz 66.4 Kb (ftp)(http) GPR
Processed data included within Sample table

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