|
| Status |
Public on Jul 27, 2014 |
| Title |
ago5-2_Digital_Gene_Indexing_Rep2 |
| Sample type |
SRA |
| |
|
| Source name |
ago5-2_Digital_Gene_Indexing
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
ip antibody: none
tissue: Mixed Inflorescence
|
| Growth protocol |
All plants were grown at 20°C in 16 hour day lengths. Mixed Inflorescence tissue was harvested approximately 6-7 weeks post germination.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted using Trizol Reagent. PolyA RNA cloned using Illumina Digital Gene Expression- Taq profiling for NlaIII
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Digital Gene Indexing
|
| Data processing |
Alignment: Sequence reads were obtained and mapped to the Arabidopsis thaliana genome (TAIR9). Only reads with 100% match were retained.
|
| |
|
| Submission date |
Jul 27, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Krys Kelly |
| Organization name |
University of Cambridge
|
| Department |
Plant Sciences
|
| Lab |
Baulcombe Group
|
| Street address |
Downing Street
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 3EA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL9302 |
| Series (1) |
| GSE30970 |
Arabidopsis AGO5 contributes to a POL II-based RNA silencing pathway directing epigenetic marks |
|
| Relations |
| SRA |
SRX085162 |
| BioSample |
SAMN00678199 |
