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Sample GSM739214 Query DataSets for GSM739214
Status Public on Jun 09, 2011
Title Blood, biological rep1
Sample type RNA
 
Source name Pure blood sample
Organism Homo sapiens
Characteristics tissue: blood
gender: female
developmental stage: adult
Treatment protocol No treatment.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol and RNeasy according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human GeneChip HG-U133_Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner GCS3000.
Description Exploratory expression profiling.
Data processing The data were analyzed with Robust Multiarray Average (RMA) normalization and scaled to a 2% trimmed mean of 150 using R/Bioconductor.
 
Submission date Jun 08, 2011
Last update date Jun 09, 2011
Contact name Ting Gong
Organization name Novartis Institutes for Biomedical research
Street address 45 Sidney street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL570
Series (1)
GSE29832 Expression data from pure/mixed blood and breast to test feasability of deconvolution of clinical samples

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 251.1479892
1053_at 126.5507729
117_at 1209.2871
121_at 365.5084574
1255_g_at 14.68219932
1294_at 224.4643072
1316_at 96.11263877
1320_at 66.24455833
1405_i_at 2700.365517
1431_at 13.06014276
1438_at 84.67151456
1487_at 151.9360414
1494_f_at 56.84354453
1552256_a_at 95.64162628
1552257_a_at 116.2427519
1552258_at 65.86626559
1552261_at 75.53308799
1552263_at 415.6468973
1552264_a_at 681.9471307
1552266_at 32.32440611

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM739214.cel.gz 8.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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