|
| Status |
Public on Jun 30, 2011 |
| Title |
NA18517_1 genotyping at TCAG (G4423B 018897) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Input DNA from Coriell Cell Repositories
|
| Organism |
Homo sapiens |
| Characteristics |
batch: batcha1a2
site: TCAG
|
| Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA18517
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was obtained from Coriell Cell Repositories, extracted from lymphoblastoid cell lines.
|
| Label |
c3
|
| Label protocol |
Invitrogen BioPrime CGH labeling kit was used (cat. No. 18094011), according to the manufactures protocol.
|
| |
|
| Channel 2 |
| Source name |
GM10851
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: GM10851
|
| Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM10851
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was obtained from Coriell Cell Repositories, extracted from lymphoblastoid cell lines.
|
| Label |
GM10851
|
| Label protocol |
Invitrogen BioPrime CGH labeling kit was used (cat. No. 18094011), according to the manufactures protocol.
|
| |
|
| |
| Hybridization protocol |
On each slide, a total combined 55ug of DNA was hybridized according to the 40-hour manual protocol described by manufacturer.
|
| Scan protocol |
Arrays were scanned at 5 micron resolution in an Agilent Scanner
|
| Data processing |
Images were processed with Agilent Feature Extraction v9.5.1.1, and were required to show derivative Log Ratio spread (DLRS) values < 0.3. CNV detection was performed using Agilent's ADM-2 and also with the Nexus rank segmentation software.
|
| |
|
| Submission date |
Mar 25, 2011 |
| Last update date |
Sep 06, 2011 |
| Contact name |
Stephen W Scherer |
| E-mail(s) |
stephen.scherer@sickkids.ca
|
| Organization name |
The Hospital for Sick Children
|
| Department |
Genetics and Genomic Biology
|
| Street address |
686 Bay Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6944 |
| Series (2) |
| GSE25893 |
A comprehensive assessment of array-based platforms and calling algorithms for detection of copy number variants |
| GSE28187 |
A comprehensive assessment of array-based platforms and calling algorithms for detection of copy number variants (2X244K) |
|
