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Sample GSM682308 Query DataSets for GSM682308
Status Public on Feb 28, 2011
Title ubb_d14_wt_1
Sample type RNA
 
Source name 14dpp wild type testis
Organism Mus musculus
Characteristics age: 14dpp
genotype/variation: wild type
tissue: testis
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
 
Hybridization protocol Fragmented, biotin labeled cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data from 14dpp wild type testis.
Data processing The data were analyzed with Microarray Suite 5.0 using a scaling factor of 125 and imported into Genespring 7.0.
 
Submission date Feb 28, 2011
Last update date Feb 28, 2011
Contact name Ryan Evanoff
E-mail(s) revanoff@wsu.edu
Phone 509-335-2240
Organization name Washington State University
Department School of Molecular Biosciences
Lab Griswold Lab
Street address PO Box 647520
City Pullman
State/province WA
ZIP/Postal code 99164-7520
Country USA
 
Platform ID GPL1261
Series (1)
GSE27568 Ubb Knockout Mouse Testis

Data table header descriptions
ID_REF
VALUE MAS 5.0 raw expression values, detection calls, and detection p-values.
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 458.5 P 0.002
1415671_at 526.1 P 0.002
1415672_at 1338.9 P 0.002
1415673_at 414.7 P 0.002
1415674_a_at 525.8 P 0.002
1415675_at 232.8 P 0.002
1415676_a_at 1048.2 P 0.002
1415677_at 164.1 P 0.002
1415678_at 634.2 P 0.002
1415679_at 1062.8 P 0.002
1415680_at 646.8 P 0.002
1415681_at 566.1 P 0.002
1415682_at 290.4 P 0.002
1415683_at 503.5 P 0.002
1415684_at 209.9 P 0.002
1415685_at 432.0 P 0.002
1415686_at 534.5 P 0.002
1415687_a_at 2136.2 P 0.002
1415688_at 882.2 P 0.002
1415689_s_at 162.1 P 0.002

Total number of rows: 45101

Table truncated, full table size 1076 Kbytes.




Supplementary file Size Download File type/resource
GSM682308.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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