|
Status |
Public on Feb 28, 2011 |
Title |
ubb_d14_wt_1 |
Sample type |
RNA |
|
|
Source name |
14dpp wild type testis
|
Organism |
Mus musculus |
Characteristics |
age: 14dpp genotype/variation: wild type tissue: testis
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
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|
|
Hybridization protocol |
Fragmented, biotin labeled cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Gene expression data from 14dpp wild type testis.
|
Data processing |
The data were analyzed with Microarray Suite 5.0 using a scaling factor of 125 and imported into Genespring 7.0.
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|
|
Submission date |
Feb 28, 2011 |
Last update date |
Feb 28, 2011 |
Contact name |
Ryan Evanoff |
E-mail(s) |
revanoff@wsu.edu
|
Phone |
509-335-2240
|
Organization name |
Washington State University
|
Department |
School of Molecular Biosciences
|
Lab |
Griswold Lab
|
Street address |
PO Box 647520
|
City |
Pullman |
State/province |
WA |
ZIP/Postal code |
99164-7520 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
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