|
| Status |
Public on Jan 19, 2011 |
| Title |
tkv[k19713]_2_2 (1631824011-C2) |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila wing imaginal disc
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Samarkand
line number: 11191
allele: tkv[k19713]
developmental stage: Third instar larvae
tissue: Wing imaginal disc
biological replicate: 2
technical (labeling) replicate: 2
sentrix_id (slide): 1631824011
sentrix position (array): C2
hybridization date: June 02 2007
|
| Growth protocol |
Larvae for each genotype were grown in bottles, and late third instar larvae were collected, sexed, and the wing imaginal discs were dissected out.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using a modified protocol for the RNAeasy kit (Qiagen), with DNase I treatment. We Used ~ 40-50 imaginal discs for each biological replicate
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA amplification kit (ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using BeadStudio version 3.0.14 ( llumina Inc)
|
| Description |
1631824011-C2
|
| Data processing |
The data were median normalised using custom R scripts
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Jan 19, 2011 |
| Contact name |
Ian Dworkin |
| Organization name |
Michigan State University
|
| Department |
Zoology
|
| Street address |
203 Natural Science Bldg. Michigan State University
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
|
| Platform ID |
GPL7740 |
| Series (1) |
| GSE26706 |
The effects of weak genetic perturbations on the transcriptome of the wing imaginal disc, and its association with wing shape in Drosophila melanogaster |
|
