|
| Status |
Public on Jan 01, 2014 |
| Title |
M1-type bone marrow derived macrophage Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse bone marrow cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 6-8 weeks
Sex: Female
tissue: bone marrow cells
|
| Treatment protocol |
Macrophages were stimulated with LPS (100ng/ml, 14 hours) and INF-g (10ng/ml, 6 hours)
|
| Growth protocol |
mouse bone marrow derived macrophages were cultured in DMEM-F12/10% FCS/10% L929-conditioned medium, at 37oC in a humidified atmosphere of 5% Co3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mirVana miRNA Isolation Kit, see http://www.ambion.com/catalog/CatNum.php?1560
|
| Label |
N/A
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
N/A
|
| Scan protocol |
N/A
|
| Description |
Biological replicate 2 of 3. M1-type macrophage cells.
|
| Data processing |
Taqman low density arrays were run on the 7900 HT Sequence Detection system. The ABI TaqMan SDS v2.3 software was utilized to obtain raw CT values. To review results, the raw CT data (SDS file format) were exported from the Plate Centric View into the ABI TaqMan RQ manager software. Automatic baseline and manual CTs were calculated with the RQ Manager Software for all samples. Statistical analysis: The global data set of miRs from card A and B was used for analysis. Data were calculated using Real Time StatMiner Tools [Integromics. http://www.integromics.com/StatMiner.php]. Input data for analysis consisted of all samples. CT values were normalized to the most stable endogenous controls (snoRNA202 and MammU6 for Card A and B) according to the Genorm algorithm.
|
| |
|
| Submission date |
Jan 11, 2011 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Andreas Schober |
| E-mail(s) |
aschober@med.lmu.de
|
| Organization name |
Ludwig-Maximilians-University
|
| Department |
Institute for Cardiovascular Prevention
|
| Street address |
Pettenkoferstr. 9
|
| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11419 |
| Series (2) |
| GSE26556 |
MiR expression profile in M1-polarized bone marrow derived macrophage cells compared with normal mouse aortic tissue. |
| GSE26731 |
MicroRNA signature in murine atherosclerosis |
|
