|
| Status |
Public on Nov 12, 2010 |
| Title |
ChIP-Seq analysis of H3K27me3 in human hSKM cells; Lib:MC:20100222:12--ChIP:MC:20100216:12:hSKM:H3K27Me3 |
| Sample type |
SRA |
| |
|
| Source name |
"Expanded from muscle spindle cells (Muoio et al; Diabetes 51, 901 (2002))"; Lib:MC:20100222:12--ChIP:MC:20100216:12:hSKM:H3K27Me3
|
| Organism |
Homo sapiens |
| Characteristics |
lineage: mesoderm
medium: DMEM
molecule: genomic DNA
disease: none
passage: unknown
line: hSKM
differentiation_method: in vivo
batch: hSKM-3
biomaterial_type: Cell Line
differentiation_stage: differentiated
Sex: Unknown
biomaterial_provider: Duke University
chip_protocol_bead_type: Protein A-Sepharose 4B
chip_antibody: H3K27Me3
chip_protocol_chromatin_amount: 10 million cells
extraction_protocol: SDS lysis
chip_protocol_antibody_amount: 2-15 ug
chip_protocol_bead_amount: 70 ul bed volume
chip_protocol: Bernstein_BROAD_ENCODE_protocol
extraction_protocol_sonication_cycles: 1
chip_antibody_catalog: 07-449
chip_antibody_provider: Millipore
chip_antibody_lot: DAM1514011
experiment_type: Histone H3K27me3
extraction_protocol_type_of_sonicator: Bioruptor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
sample_term_id: CL_0000594
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: Lib:MC:20100222:12--ChIP:MC:20100216:12:hSKM:H3K27Me3
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.3179
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.3211
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
BED and WIG files were replaced on April 29, 2014
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM621755_BI.Muscle_Satellite_Cultured_Cells.H3K27me3.hSKM-3.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: Lib:MC:20100222:12--ChIP:MC:20100216:12:hSKM:H3K27Me3.hg19.level.1
ANALYSIS TITLE: Mapping of Muscle Satellite Cultured Cells Histone H3K27me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by Histone H3K27me3 ChIP-Seq on Muscle Satellite Cultured Cells, Donor hSKM-3 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.4601
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 2
QUALITY SCORES:
NUMBER_OF_H3K27me3_EXPERIMENTS_SCORED: 52
FINDPEAKS_SCORE: 0.1041
FINDPEAKS_PERCENTILE: 42
HOTSPOT_SCORE: 0.173
HOTSPOT_PERCENTILE: 40
IROC_SCORE: 0.8881
IROC_PERCENTILE: 38
POISSON_SCORE: 0.543
POISSON_PERCENTILE: 83
**********************************************************************
ANALYSIS FILE NAME: GSM621755_BI.Muscle_Satellite_Cultured_Cells.H3K27me3.hSKM-3.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: Lib:MC:20100222:12--ChIP:MC:20100216:12:hSKM:H3K27Me3.hg19.level.2
ANALYSIS TITLE: Raw Signal Density Graphs of Muscle Satellite Cultured Cells Histone H3K27me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina Histone H3K27me3 ChIP-Seq read mappings from Muscle Satellite Cultured Cells, Donor hSKM-3 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.4875
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 2
BROWSER_TRACK_NAME: MS H3K27me3 3
BROWSER_TRACK_DESCRIPTION: BI Muscle Satellite Cultured Cells Histone H3K27me3 Donor hSKM-3 EA Release 2
QUALITY SCORES:
NUMBER_OF_H3K27me3_EXPERIMENTS_SCORED: 52
FINDPEAKS_SCORE: 0.1041
FINDPEAKS_PERCENTILE: 42
HOTSPOT_SCORE: 0.173
HOTSPOT_PERCENTILE: 40
IROC_SCORE: 0.8881
IROC_PERCENTILE: 38
POISSON_SCORE: 0.543
POISSON_PERCENTILE: 83
**********************************************************************
|
| |
|
| Submission date |
Nov 10, 2010 |
| Last update date |
Jan 29, 2015 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (1) |
| GSE25249 |
BI Human Reference Epigenome Mapping Project: Characterization of chromatin modification by ChIP-Seq in human subject |
|
| Relations |
| BioSample |
SAMN00119994 |
| Named Annotation |
GSM621755_BI.Muscle_Satellite_Cultured_Cells.H3K27me3.hSKM-3.wig.gz |
