NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM618097 Query DataSets for GSM618097
Status Public on Apr 11, 2011
Title BPN, 6 weeks old, 6
Sample type RNA
 
Source name Male BPN/3J hypothalamus, 6 weeks old
Organism Mus musculus
Characteristics strain: BPN/3J
gender: male
age: 6 weeks
tissue: hypothalamus
blood pressure: normotensive
Treatment protocol Standard animal house treatment.
Growth protocol Hypothalamus was removed from BPH/2J and BPN/3J strains of mice at peak blood pressure. Tissues were frozen and then RNA was extracted.
Extracted molecule total RNA
Extraction protocol The RNeasy kit (Qiagen) was used for extraction of total RNA according to the manufacturer's instructions.
Label biotin
Label protocol 100ng of total RNA was prepared for hybridisation according to the standard Affymetrix protocol (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual, version 4, Affymetrix).
 
Hybridization protocol After fragmentation, ssDNA was hybridized for 17 hr at 45°C to GeneChip Mouse Gene 1.0 ST Arrays. Chips were washed and stained in the Affymetrix GeneChip® Fluidics Stations 450. For all of these steps, the GeneChip Hybridization, Wash, and Stain Kit (Affymetrix) was used according to the manufacturer’s recommendations (GeneChip® Whole Transcript (WT) Sense Target Labelling Assay Manual, version 4, Affymetrix).
Scan protocol The chips were scanned using the GeneChip® Scanner 3000 7G 4C (Affymetrix) according to the manufacturer’s recommendations (GeneChip® Whole Transcript (WT) Sense Target Labelling Assay Manual, version 4, Affymetrix).
Description BPN12Y
Gene expression from young normotensive BPN/3J mouse.
Data processing The samples were individually normalized using robust-multi-array analysis (RMA) via the package 'Affy' in the R application (version 2.11.1). Differentially expressed genes for each age were identified using the moderated two-sample t-test in the Limma package.
 
Submission date Nov 04, 2010
Last update date Apr 11, 2011
Contact name Prof.Brian J Morris
E-mail(s) brian.morris@sydney.edu.au
Organization name University of Sydney
Department Physiology
Lab Basic & Clinical Genomics Lab
Street address Anderson Stuart Building, room N452, University of Sydney
City Sydney
State/province NSW
ZIP/Postal code 2006
Country Australia
 
Platform ID GPL6246
Series (1)
GSE25076 Hypothalamic expression differences between hypertensive BPH/2J and normotensive BPN/3J mouse strains

Data table header descriptions
ID_REF
VALUE RMA-normalized log2 expression value

Data table
ID_REF VALUE
10338001 12.41792252
10338003 10.51672116
10338004 9.43798885
10338017 13.1274212
10338025 9.117907652
10338026 13.51347192
10338029 9.828899765
10338035 9.113474664
10338036 9.678328208
10338037 3.566381965
10338041 11.65003222
10338042 10.34727801
10338044 12.47102003
10338047 7.041309688
10338056 3.232559619
10338059 13.48527561
10338060 3.68533419
10338063 3.338306327
10338064 6.365455389
10338065 6.862897765

Total number of rows: 34760

Table truncated, full table size 709 Kbytes.




Supplementary file Size Download File type/resource
GSM618097.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap