|
| Status |
Public on Feb 11, 2011 |
| Title |
c24floral-23-rep2 |
| Sample type |
SRA |
| |
|
| Source name |
floral tissue
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: C24
tissue: floral
developmental stage: stage 1-14
|
| Growth protocol |
23°C, 16 hour light period
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Libraries were constructed as per (Mosher RA et al., 2009), except small RNA were isolated by PAGE, and RNA amplicons were reverse transcribed using the Fermentas Revertaid kit (Fermentas Life Sciences) and then amplified by PCR using the Phusion DNA polymerase (Finnzymes).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
small RNA
|
| Data processing |
Sequencing adapters were removed and sequences without adapter were discarded. The txt files list unique sequences together with their counts.
|
| |
|
| Submission date |
Oct 07, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Felix Ott |
| Organization name |
Max Planck Institute for Developmental Biology
|
| Street address |
Spemannstr. 35-39
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9302 |
| Series (1) |
| GSE24569 |
Homology-guided Assembly of the Genomes of the Four Diverse Accessions Ler, C24, Bur-0 and Kro-0 |
|
| Relations |
| SRA |
SRX039648 |
| BioSample |
SAMN00199214 |
