|
| Status |
Public on Apr 17, 2011 |
| Title |
HITC6, Day 8 biological replicate 3, Affy153A |
| Sample type |
RNA |
| |
|
| Source name |
HITC6 smooth muscle cells, 8 days after serum withdrawal
|
| Organism |
Homo sapiens |
| Characteristics |
time point: 8 days after serum withdrawal
cell line: human SMC line derived from the distal internal thoracic artery
|
| Treatment protocol |
cultures were rinsed twice with phosphate buffered saline and the media was replaced with M199 without fetal bovine serum
|
| Growth protocol |
cultures of SMCs were maintained in M199 supplemented with 10% fetal bovine serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
www.affymetrix.com/support/downloads/manuals/expression_analysis_technical_manual.pdf
|
| |
|
| Hybridization protocol |
www.affymetrix.com/support/downloads/manuals/expression_analysis_technical_manual.pdf
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression information from HITC6 SMCs at Day 8 after serum withdrawal
|
| Data processing |
The data were analyzed with Expression Console version 1.1 using the MAS 5.0 algorithm and scaled all probe sets to a target value of 150.
|
| |
|
| Submission date |
Apr 16, 2010 |
| Last update date |
Apr 17, 2011 |
| Contact name |
J. Geoffrey Pickering |
| E-mail(s) |
gpickering@robarts.ca
|
| Organization name |
Robarts Research Institute
|
| Street address |
100 Perth Drive
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A5K8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE21363 |
Gene expression during SMC cord morphogenesis |
|
