|
| Status |
Public on Feb 15, 2010 |
| Title |
MCF10A |
| Sample type |
genomic |
| |
|
| Source name |
cell line MCF10A
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF10A
cell type: non-tumorigenic breast cell line MCF10A
|
| Treatment protocol |
None
|
| Growth protocol |
DMEM/F12 medium supplemented with 5% horse serum, 10 μg/ml insulin, 20 ng/ml EGF, 0.5 μg/ml hydrocortisone, and 100 ng/ml cholera toxin
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated using the Qiagen Blood and Cell Culture midi Kit.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As per manufacturer (Affymetrix)
|
| Scan protocol |
Arrays were washed and stained in the Genechip Fluidics Station 450 (Affymetrix) following the manufacturer’s instructions. Chips were scanned with the Affymetrix GeneChip Scanner 3000 using GeneChipOperating System 1.4
|
| Description |
Hybridized to 250K_Sty
|
| Data processing |
*.CEL files were normalized using Affymetrix Power Tools version 1.85 gtype-probeset-genotype program. Copy number was determined using the Affymetrix Power Tools version 1.85 copynumber-pipeline program.
|
| |
|
| Submission date |
Jan 15, 2010 |
| Last update date |
Jan 15, 2010 |
| Contact name |
Robert J Clifford |
| E-mail(s) |
clifforr@mail.nih.gov
|
| Phone |
301-435-1527
|
| Fax |
301-480-6641
|
| Organization name |
NCI
|
| Department |
CCR
|
| Lab |
LPG
|
| Street address |
2115 E Jefferson
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platform ID |
GPL3720 |
| Series (1) |
| GSE19920 |
Affymetrix SNP array analysis of the breast cell lines MCF10A, MCF10AT, MCF10CA1h and MCF10CA1a |
|
