|
Status |
Public on Jul 17, 2009 |
Title |
mvfR mutant constitutively expressing PqsE from a plasmid rep1 |
Sample type |
RNA |
|
|
Source name |
Pseudomonas aeruginosa PA14 mvfR mutant constitutively expressed PqsE
|
Organism |
Pseudomonas aeruginosa |
Characteristics |
bacteria: Pseudomonas aeruginosa strain: PA14 mutant: mvfR plasmid: pDN19pqsE, tetracycline resistant
|
Growth protocol |
Cells were inoculated from -80C stock, grown in LB with tetracycline (120ug/ml) at 37C over night, diluted 1:100 and grown to OD600nm of 3.0
|
Extracted molecule |
total RNA |
Extraction protocol |
According to Qiagen RNAeasy mini kit
|
Label |
biotin
|
Label protocol |
According to Enzo Life sciences BioArray terminal labeling kit with biotin ddUTP.
|
|
|
Hybridization protocol |
According to Affymetrix GeneChip® Fluidics Station 450 protocol.
|
Scan protocol |
According to Affymetrix scanner 3000 protocol
|
Description |
PA14 mvfR mutant was transformed with the plasmid pDN19pqsE that contain a constitutive expressed PqsE under ptac promoter.
|
Data processing |
Affymetrix Microarray Suite version 5.0
|
|
|
Submission date |
Jul 15, 2009 |
Last update date |
Jul 16, 2009 |
Contact name |
Laurence G Rahme |
E-mail(s) |
rahme@molbio.mgh.harvard.edu
|
Phone |
617-724-5003
|
Fax |
617-724-8558
|
Organization name |
Massachusetts General Hospital
|
Department |
Surgery
|
Street address |
50 Blosson St, Thier 340
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02114 |
Country |
USA |
|
|
Platform ID |
GPL84 |
Series (2) |
GSE17116 |
Pseudomonas aeruginosa PA14, pqsE and PQS |
GSE17147 |
Pseudomonas aeruginosa PA14 MvfR-dependent QS regulatory pathway |
|