strain: C57BL/6 tissue: whole embryo stage: E18.5 genotype: ER-alpha KO
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from embryo using TRIzol (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions.
Label
FAM
Label protocol
Standard TaqMan protocol
Hybridization protocol
n/a
Scan protocol
n/a
Description
Standard Taqman Assay: Quantitative real-time PCR was performed using Applied Biosystems 7900HT system and universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles).
Data processing
Data was processed using SDS 2.3 software (Applied Biosystems, Foster City, CA). Normalized by subtracting mammu6 to get Delta CT