|
| Status |
Public on Feb 21, 2009 |
| Title |
Hek-HT Control Replicate-3 |
| Sample type |
RNA |
| |
|
| Source name |
THX-3
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: transformed Hek cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We extracted total RNA using Qiagen Rneasy mini kits.
|
| Label |
biotin
|
| Label protocol |
We performed an in vitro transcription reaction to produce a biotin-labeled cRNA from the cDNA.
|
| |
|
| Hybridization protocol |
The cRNA is fragmented before hybridization and a hybridization cocktail is prepared that contains the fragmented cRNA, probe array controls, BSA and herring sperm DNA. The cRNA is hybridized to the oligonucleotide probes on the array for 16 hours at 45C.
|
| Scan protocol |
Immediately following hybridization, the hybridized probe arrays undergo an automated washing and staining protocol on the fluidic station and are then scanned on the GeneChip Scanner 3000 7G.
|
| Description |
1462_2979_14859_THX-3_HG-U133A+2.CEL
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Feb 20, 2009 |
| Last update date |
Jun 22, 2021 |
| Contact name |
Jeffrey Chang |
| E-mail(s) |
jeffrey.t.chang@uth.tmc.edu
|
| Organization name |
University of Texas
|
| Street address |
6431 Fannin St
|
| City |
Houston |
| ZIP/Postal code |
77019 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE14934 |
Gene expression profiles of Ras mutants. |
|
