Strain: Wild-type, C57Bl/6 background Gender: female Age: 28 days Eye: Left
Biomaterial provider
Clark lab mouse colony
Treatment protocol
Lens was cryosectioned, stained with Cressyl Violet, and dehydrated. Laser capture microdissection (Arcturus PixCell2e) was used to remove epithelial cells from each section.
Growth protocol
Normal housing
Extracted molecule
total RNA
Extraction protocol
RNeasy micro kit (Qiagen)
Label
Biotin
Label protocol
Two cycle amplification using Ambion MessageAmp II aRNA amplification kit and MessageAmp II-biotin enhanced amplification kits
Hybridization protocol
GeneChip Hybridization Oven at 45ÂșC with rotation, washing on GeneChip Fulidics Station_450 using EukGE-Ws2v4_450 washing/staining protocol with Antibody Amplification Staining Protocol
Scan protocol
7G GeneChip Scanner, single scan, 570 nm filter, software GCOS1.4
Description
Each data set was taken from one lens only. All lens data sets were from one litter of animals.
Data processing
Global scaling in GCOS1.4 using all probsets and TGT value of 500