|
| Status |
Public on Oct 09, 2008 |
| Title |
Sample 2 primary neuroblastoma |
| Sample type |
RNA |
| |
|
| Source name |
primary tumour
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: male
Age: 4 years
Stage: 4
MYCN amp.:no
1p del: no
11q del: yes
17q gain: yes
|
| Treatment protocol |
Tumour samples were snap frozen and stored at -80oC prior to RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Micro kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix Protocol
|
| |
|
| Hybridization protocol |
Biotinylated and fragmented cRNA (15 ug) was hybridised to the GeneChip arrays at 45oC for 16 hours, washed, and stained with streptavidin phycoerythrin using an Affymetrix FS-450 fluidics station.
|
| Scan protocol |
Scanned using Genechip Scanner 3000
|
| Description |
none
|
| Data processing |
Raw data were firstly scaled using GCOS 1.1.1 software to a target signal of 500. Data were then imported into GeneSpring GX (Agilent Technologies, Santa Clara, CA, USA) when a ‘per gene normalisation’ to the median expression level of the gene across all samples was performed.
|
| |
|
| Submission date |
Oct 09, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Michael Stewart Jackson |
| E-mail(s) |
m.s.jackson@ncl.ac.uk
|
| Phone |
+44 191 241 8677
|
| Organization name |
Newcastle University
|
| Department |
Institute of Genetic Medicine
|
| Street address |
Central Parkway
|
| City |
Newcastle upon Tyne |
| ZIP/Postal code |
NE1 3BZ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE13136 |
Identification of candidate neuroblastoma genes by combining genomic and expression microarrays: expression data |
| GSE13141 |
Identification of candidate neuroblastoma genes by combining genomic and expression microarrays |
|
| Relations |
| Reanalyzed by |
GSE119087 |
