Characteristics S. cerevisiae prototrophic haploid reference strain CEN.PK113-7D (MATa)
Biomaterial provider
JM Daran
Treatment protocol
Culture quenched in liquid nitrogen
Growth protocol
The S. cerevisiae prototrophic haploid reference strain CEN.PK113-7D (MATa) was grown at a dilution rate (D) of 0.03 h-1 at 30oC in 2-liter chemostats (Applikon, Schiedam, The Netherlands) with a working volume of 1.0 liter as described in {van den Berg, 1996;Tai, 2005}. A temperature probe connected to a cryostat controlled cultures grown at 30oC. Cultures were grown in a defined synthetic medium that was limited by nitrogen with all other growth requirements in excess as described by {Tai, 2005}. The dilution rate was set at 0.03 hr-1 with pH measured online and kept constant at 5.0 by automatic addition of 2 M KOH using an Applikon ADI 1030 Biocontroller. The stirrer speed was set to 600 rpm. Anaerobic growth and steady state conditions were maintained as described {Tai, 2005}. Biomass dry weight, metabolite, dissolved oxygen, and gas profiles were constant at least three volume changes prior to sampling. Tai, S. L., Boer, V. M., Daran-Lapujade, P., Walsh, M. C., de Winde, J. H., Daran, J. M. & Pronk, J. T. (2005) J. Biol. Chem. 280, 437-447. van den Berg, M. A., de Jong-Gubbels, P., Kortland, C. J., van Dijken, J. P., Pronk, J. T. & Steensma, H. Y. (1996) J. Biol. Chem. 271, 28953-28959.
Extracted molecule
total RNA
Extraction protocol
Sampling of cells from chemostats, probe preparation, and hybridization to Affymetrix Genechip® microarrays were performed as recommended by Affymetrix
Sampling of cells from chemostats, probe preparation, and hybridization to Affymetrix Genechip® microarrays were performed as recommended by Affymetrix
Hybridization protocol
Sampling of cells from chemostats, probe preparation, and hybridization to Affymetrix Genechip® microarrays were performed as recommended by Affymetrix
