|
| Status |
Public on May 03, 2008 |
| Title |
22 h post LH surge, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Granulosa cells collected 22 h post LH surge from buffalo cows
|
| Organism |
Bubalus bubalis |
| Characteristics |
Granulosa cells collected 22 h post LH surge
|
| Biomaterial provider |
Dr. R. Medhamurthy
|
| Treatment protocol |
For the purpose of microarray analysis, GnRH (100 µg, i.m.) was administered 36 h post PGF2alpha, Ovaries were collected at 24 h post GnRH, from which granulosa cells were isolated (n=3).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol reagent (Sigma Aldrich Co., St.Louis, MO) according to manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev. 3, 2001)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev. 3, 2001)
|
| Scan protocol |
Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev. 3, 2001) using scanner 3000 series
|
| Description |
Probe level measurements produced by robust multichip average (RMA) algorithm
|
| Data processing |
The processed image files (.CEL) were normalized across arrays using the robust multichip average (RMA) algorithm and log-transformed (base 2).
|
| |
|
| Submission date |
May 01, 2008 |
| Last update date |
May 02, 2008 |
| Contact name |
jyotsna U rao |
| E-mail(s) |
jyothsna@mrdg.iisc.ernet.in
|
| Phone |
9180-22932490
|
| Fax |
9180-23600999
|
| Organization name |
Indian Institute of Science
|
| Department |
MRDG
|
| Lab |
Dr. R. Medhamurthy
|
| Street address |
C.V. Raman Road
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560012 |
| Country |
India |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE11312 |
Gene expression profiling of the periovulatory follicle |
|
