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Status |
Public on Sep 22, 2017 |
Title |
AML_NPM1wt_pat4 |
Sample type |
RNA |
|
|
Source name |
enriched leukemic blast cells
|
Organism |
Homo sapiens |
Characteristics |
disease state: AML genotype/variation: NPM1 wildtype relative hsa_circ_0075001 expression: high cell type: enriched leukemic blast cells
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA was purified with AllPrep DNA/RNA kits (QIAGEN).
|
Label |
biotin
|
Label protocol |
For each sample preparation, total RNA was converted into double-stranded cDNA by reverse transcription using the 3' IVT Express Kit from Affymetrix according to the manufacturer's recommendations.
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|
|
Hybridization protocol |
Hybridization, washing, and staining protocols, respectively, were performed on Affymetrix GeneChip instruments (Hybridization Oven 640, Fluidics Station FS450) as recommended by the manufacturer.
|
Scan protocol |
Scanning was performed on Affymetrix GeneChip Scanner GCS3000 instruments as recommended by the manufacturer using default settings. The software used was GCOS 1.2 or higher.
|
Description |
Gene expression data from AML with normal karyotype
|
Data processing |
The data were analyzed with PartekĀ® Genomics SuiteĀ® 6.6 using RMA, which includes normalization and log2 transformation of intensity values.
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|
|
Submission date |
Sep 21, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Lars Bullinger |
E-mail(s) |
lars.bullinger@uniklinik-ulm.de
|
Organization name |
University of Ulm
|
Department |
Internal Medicine III
|
Street address |
Albert-Einstein-Allee 23
|
City |
Ulm |
ZIP/Postal code |
89081 |
Country |
Germany |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE104099 |
Circular RNAs of the nucleophosmin (NPM1) gene in acute myeloid leukemia |
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