NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2789770 Query DataSets for GSM2789770
Status Public on Sep 22, 2017
Title AML_NPM1wt_pat4
Sample type RNA
 
Source name enriched leukemic blast cells
Organism Homo sapiens
Characteristics disease state: AML
genotype/variation: NPM1 wildtype
relative hsa_circ_0075001 expression: high
cell type: enriched leukemic blast cells
Extracted molecule total RNA
Extraction protocol The total RNA was purified with AllPrep DNA/RNA kits (QIAGEN).
Label biotin
Label protocol For each sample preparation, total RNA was converted into double-stranded cDNA by reverse transcription using the 3' IVT Express Kit from Affymetrix according to the manufacturer's recommendations.
 
Hybridization protocol Hybridization, washing, and staining protocols, respectively, were performed on Affymetrix GeneChip instruments (Hybridization Oven 640, Fluidics Station FS450) as recommended by the manufacturer.
Scan protocol Scanning was performed on Affymetrix GeneChip Scanner GCS3000 instruments as recommended by the manufacturer using default settings. The software used was GCOS 1.2 or higher.
Description Gene expression data from AML with normal karyotype
Data processing The data were analyzed with PartekĀ® Genomics SuiteĀ® 6.6 using RMA, which includes normalization and log2 transformation of intensity values.
 
Submission date Sep 21, 2017
Last update date Jan 23, 2018
Contact name Lars Bullinger
E-mail(s) lars.bullinger@uniklinik-ulm.de
Organization name University of Ulm
Department Internal Medicine III
Street address Albert-Einstein-Allee 23
City Ulm
ZIP/Postal code 89081
Country Germany
 
Platform ID GPL570
Series (1)
GSE104099 Circular RNAs of the nucleophosmin (NPM1) gene in acute myeloid leukemia

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
1007_s_at 3.09546
1053_at 4.45973
117_at 3.53183
121_at 5.61688
1255_g_at 1.85706
1294_at 5.92317
1316_at 3.63586
1320_at 2.78138
1405_i_at 6.71762
1431_at 2.17379
1438_at 2.96385
1487_at 4.91451
1494_f_at 3.70185
1552256_a_at 4.70608
1552257_a_at 4.45894
1552258_at 3.60479
1552261_at 3.04847
1552263_at 3.71685
1552264_a_at 5.6258
1552266_at 1.64225

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM2789770_4_NPM1wt.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap