|
| Status |
Public on Mar 09, 2009 |
| Title |
N8456B1: Unremarkable breast ducts (Normal) |
| Sample type |
RNA |
| |
|
| Source name |
frozen breast tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Unremarkable breast ducts (Normal)
|
| Treatment protocol |
The tissues were embedded in Tissue-Tek® O.C.T., 5-um sections cut. The sections were stained using a standard H&E protocol, and tissue boundaries marked by a pathologist Using the marked slide as a map, tissues were microdissected. Adipose tissues were trimmed, the tumor and normal tissues were separated and stored in liquid nitrogen.
|
| Growth protocol |
Breast tissues (normal and malignant) from patients that underwent mastectomy for various stages of breast carcinoma were collected and frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from microdissected breast tissues using the Trizol method.
|
| Label |
biotin
|
| Label protocol |
Labeling was done according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization was carried out according to the standard Affymetrix protocol
|
| Scan protocol |
According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array)
|
| Description |
frozen breast tissue
|
| Data processing |
Expression values were calculated using the robust multi-array average (RMA) algorithm
|
| |
|
| Submission date |
Mar 11, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Dung-Tsa Chen |
| E-mail(s) |
dung-tsa.chen@moffitt.org
|
| Organization name |
Moffitt Cancer Center
|
| Department |
Biostatistics and Bioinformatics
|
| Street address |
12902 Magnolia Drive
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33647 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE10780 |
Proliferative genes dominate malignancy-risk gene signature in histologically-normal breast tissue |
|
| Relations |
| Reanalyzed by |
GSE119087 |
