|
| Status |
Public on Feb 14, 2008 |
| Title |
end of the night A |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis rosette at the end of the night
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Genotype: Columbia-0
Age: 5-6 weeks
Tissue: rosettes
|
| Treatment protocol |
Plants were harvested in the vegetative state with 3 rosettes per sample, and typically at least 5 replicate samples. Rosettes were transferred into liquid nitrogen in darkness.
|
| Growth protocol |
Plants were germinated for 7 days (16 h light at 20°C, night at 6°C, 200 µE fluorescent light, 60–70% relative humidity), transferred to short-day condition (8 h light at 20°C, dark at 16°C, 180 µE fluorescent light, 60–70% relative humidity), picked after day 14 into pots and incubated under short day further 7 days. The Col-0 ecotype grew from day 22 on for 11 days in a 14-h photoperiod (20°C, 150 µE fluorescent light, 60–70% relative humidity)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction
|
| Label |
biotin
|
| Label protocol |
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedure
|
| Description |
Gene expression data from Arabidopsis rosettes at the end of the night
|
| Data processing |
The data were analyzed with Bioconductor using the RMA normalization procedure
|
| |
|
| Submission date |
Dec 22, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Björn Usadel |
| E-mail(s) |
usadel@mpimp-golm.mpg.de
|
| Organization name |
Max Planck Institute of Molecular Plant Physiology
|
| Street address |
Am Mühlenberg 1
|
| City |
Potsdam |
| ZIP/Postal code |
14476 |
| Country |
Germany |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE10016 |
Expression data of Arabidopsis thaliana rosettes in an extended night |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
