NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM246162 Query DataSets for GSM246162
Status Public on Dec 04, 2007
Title Exp C_3, 1mg/kg CPF
Sample type RNA
 
Source name Brain tissue from Fisher 344 male rat treated with 1mg/kg CPF
Organism Rattus norvegicus
Characteristics Sex: male
Age: adult (11-12 weeks)
Treatment protocol F344 male rats were treated with a single dose (0, 0.5, 1.0, 5.0, 10, 30, or 50mg/kg) of CPF (Sigma) in three separate experiments and sacrificed after 96 hours of exposure to CPF.
Extracted molecule total RNA
Extraction protocol Qiagen MiniRNeasy total RNA extraction was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA was prepared according to the RNA transcript labeling procedures of Enzo from 15ug of total RNA
 
Hybridization protocol After fragmentation 15ug of cRNA was hybridized for 16 hours @ 45C on GeneChip Rat Expression Array 230A. GeneChips were washed and stained in an Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Description Exp C, Animal #3, 1mg/kg CPF
Gene expression data from male F344 rats treated with a single dose of 1mg/kg of CPF for 96 hours
Data processing Data was analyzed with Data Mining Tool version 3.0 (DMT 3.0) using the following parameters: Alpha1=0.05; Alpha2=0.06; Tau=0.015; Gamma1H=0.005; Gamma1L=0.005; Gamma2H=0.006; Gamma2L=0.006; Perturbation=1.35; Scaling (All Probe Sets) Target Intensity=500
 
Submission date Dec 03, 2007
Last update date Aug 14, 2011
Contact name Victor Chan
E-mail(s) victor.chan@wpafb.af.mil, andrea.stapleton@wpafb.af.mil
Phone 9379049501
Organization name Air Force Research Laboratory
Department Applied Biotechnology Branch
Street address 2729 R St. Area B Bldg. 837
City WPAFB
State/province OH
ZIP/Postal code 45433-5707
Country USA
 
Platform ID GPL341
Series (1)
GSE9751 Expression data from brain tissue of Rattus norvegicus treated with chlorpyrifos (CPF)

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1367452_at 2589.1 P 0.000244
1367453_at 2881.7 P 0.000244
1367454_at 531.1 P 0.005859
1367455_at 2132.9 P 0.000244
1367456_at 1908.5 P 0.000244
1367457_at 1965.6 P 0.00415
1367458_at 923.1 P 0.008057
1367459_at 7294.2 P 0.000244
1367460_at 2865.5 P 0.000244
1367461_at 929.1 P 0.001953
1367462_at 3424.5 P 0.001221
1367463_at 1325.1 P 0.001221
1367464_at 1023.4 P 0.000732
1367465_at 2169.6 P 0.000244
1367466_at 1933.7 P 0.000244
1367467_at 2363.3 P 0.00415
1367468_at 495.8 P 0.005859
1367469_at 2411.7 P 0.000244
1367470_at 2291.3 P 0.000732
1367471_at 549.3 P 0.000244

Total number of rows: 15923

Table truncated, full table size 429 Kbytes.




Supplementary file Size Download File type/resource
GSM246162.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap