|
Status |
Public on Dec 04, 2007 |
Title |
Exp C_3, 1mg/kg CPF |
Sample type |
RNA |
|
|
Source name |
Brain tissue from Fisher 344 male rat treated with 1mg/kg CPF
|
Organism |
Rattus norvegicus |
Characteristics |
Sex: male Age: adult (11-12 weeks)
|
Treatment protocol |
F344 male rats were treated with a single dose (0, 0.5, 1.0, 5.0, 10, 30, or 50mg/kg) of CPF (Sigma) in three separate experiments and sacrificed after 96 hours of exposure to CPF.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen MiniRNeasy total RNA extraction was performed according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared according to the RNA transcript labeling procedures of Enzo from 15ug of total RNA
|
|
|
Hybridization protocol |
After fragmentation 15ug of cRNA was hybridized for 16 hours @ 45C on GeneChip Rat Expression Array 230A. GeneChips were washed and stained in an Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
Description |
Exp C, Animal #3, 1mg/kg CPF Gene expression data from male F344 rats treated with a single dose of 1mg/kg of CPF for 96 hours
|
Data processing |
Data was analyzed with Data Mining Tool version 3.0 (DMT 3.0) using the following parameters: Alpha1=0.05; Alpha2=0.06; Tau=0.015; Gamma1H=0.005; Gamma1L=0.005; Gamma2H=0.006; Gamma2L=0.006; Perturbation=1.35; Scaling (All Probe Sets) Target Intensity=500
|
|
|
Submission date |
Dec 03, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Victor Chan |
E-mail(s) |
victor.chan@wpafb.af.mil, andrea.stapleton@wpafb.af.mil
|
Phone |
9379049501
|
Organization name |
Air Force Research Laboratory
|
Department |
Applied Biotechnology Branch
|
Street address |
2729 R St. Area B Bldg. 837
|
City |
WPAFB |
State/province |
OH |
ZIP/Postal code |
45433-5707 |
Country |
USA |
|
|
Platform ID |
GPL341 |
Series (1) |
GSE9751 |
Expression data from brain tissue of Rattus norvegicus treated with chlorpyrifos (CPF) |
|