|
| Status |
Public on Aug 01, 2008 |
| Title |
Hikone Male_LM2Dros405M2 |
| Sample type |
RNA |
| |
|
| Source name |
adult males
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Genotype: Japan Oregon R (Hikone)
Gender: Male
|
| Growth protocol |
Flies were reared and aged at 25C 0-6hrs post-eclosion
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from adult males.
|
| Data processing |
The data were normalized with the [R] Bioconductor Suite GC-RMA.
|
| |
|
| Submission date |
Sep 24, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Dean Baker |
| Organization name |
University of Cambridge
|
| Street address |
Department of Genetics, Downing Street
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 3EH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE9149 |
Expression data from wildtype adult Drosophila males and females |
|
