NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM189640 Query DataSets for GSM189640
Status Public on Oct 01, 2007
Title Balb/c day 1, biological rep 3, tech rep 1
Sample type RNA
 
Source name Whole brain day 1
Organism Mus musculus
Characteristics Balb/C
Adult, male
Treatment protocol Cryopreserved P. berghei ANKA was thawed and passaged through naïve B6 donor mice until parasitemia in the passage animals reached approximately 10%. On day 0, mice were infected by intraperitoneal injection with 500,000 freshly isolated PbA parasitized erythrocytes. Parasitemia was monitored daily after Day 3 using thin blood smears
Growth protocol All adult male mice were housed in the same SPF facility.
Extracted molecule total RNA
Extraction protocol Brains were homogenized in Trizol Reagent and total RNA was isolated according to the manufacturers' instructions. RNA was further purified of gDNA contamination and concentrated using a RNeasyPlus Mini Kit.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
 
Hybridization protocol Following fragmentation, cRNA was hybridized to GeneChip MOE 430A 2.0 Array per Affymetrix protocols.
Scan protocol Microarrays were scanned using Affymetrix GeneChip Scanner and GeneChip Operating Software (GCOS) was used for image analysis.
Description Gene expression data from whole brain infected mouse, day 1
Data processing Background adjustment and quantile normalization across all microarrays was performed using the Robust Multichip Average algorithm (RMAExpress).
 
Submission date May 15, 2007
Last update date Oct 19, 2022
Contact name Sina Gharib
E-mail(s) sagharib@uw.edu
Organization name University of Washington
Department Medicine
Street address 850 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL8321
Series (1)
GSE7814 Transcriptional Profiling of Cerebral Malaria

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity.

Data table
ID_REF VALUE
1415670_at 357.5026188
1415671_at 1590.082605
1415672_at 994.9712301
1415673_at 83.73721317
1415674_a_at 462.61268
1415675_at 286.7774946
1415676_a_at 1335.086036
1415677_at 485.312766
1415678_at 1018.53723
1415679_at 1233.659895
1415680_at 180.2894292
1415681_at 655.8817854
1415682_at 172.999613
1415683_at 738.768734
1415684_at 109.6177791
1415685_at 110.3277578
1415686_at 641.3810861
1415687_a_at 3398.404815
1415688_at 407.0431697
1415689_s_at 61.9448741

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM189640.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap