|
| Status |
Public on Mar 12, 2008 |
| Title |
AML 3301 |
| Sample type |
RNA |
| |
|
| Source name |
Blasts and mononuclear cells, AML patient
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Acute myeloid leukemia
cell type: blasts and mononuclear cells
idh1: neg
idh2: neg
gender: male
age: 56 years
score: FAB M5
risk: cytogenetic poor
cebpa: wild type
karyotype: -5/7(q)
npm1: Neg
flt3 itd mutation: Neg
flt3 tkd mutation: Pos
n-ras mutation: Pos
k-ras mutation: Neg
evi1 expression: Pos
cebpa mutation: Neg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by lysis with guanidium isothiocyanate followed by cesium chloride gradient purification.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Affymetrix protocol.
|
| Scan protocol |
Affymetrix protocol.
|
| Description |
Blasts and mononuclear cells were purified from bone marrow or peripheral blood aspirates of AML patients. Samples contained 80-100 percent blast cells.
|
| Data processing |
MAS5 with global scaling to target value 100, followed by setting all values lower than 30 at 30, and then log2 transformation.
|
| |
|
| Submission date |
Jan 29, 2007 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Roel Verhaak |
| E-mail(s) |
rverhaak@mdanderson.org
|
| URL |
http://odin.mdacc.tmc.edu/~rverhaak/
|
| Organization name |
MD Anderson Cancer Center
|
| Department |
Bioinformatics and Comp Bio
|
| Lab |
Verhaak
|
| Street address |
1400 Pressler St
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE6891 |
Acute myeloid leukemia samples of samples =< 60yrs on HG-U133 plus 2 |
| GSE14468 |
Gene expression profiling of CEBPA double and single mutant and CEBPA wild type AML. |
|
| Relations |
| Reanalyzed by |
GSE119087 |
| Reanalyzed by |
GSE122511 |
