Strain: Oryza sativa L. cv Nipponbare transformed with pActnos/Hmz, T0 line, Tissue: leaf, Age: 75 days after germination
Growth protocol
Transgenic plants of the T0 generation from callus grown on regeneration agar plates for 6 weeks were transferred to agar plates containing 1 X Murashige and Skoog salts, 3 % (w/v) sucrose, and 50 µg mL-1 hygromycin-B (MS-agar plate) for 7 d, and then hydroponically grown until heading in an environment-controlled greenhouse with a photoperiod of 12 h light (30°C)/12 h dark (25°C).
Extracted molecule
total RNA
Extraction protocol
Total RNA was prepared with the RNeasy plant Mini Kit (Qiagen).
Label
biotin
Label protocol
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Publication: Naoya Hirose, Nobue Makita, Mikiko Kojima, Tomoe Kamada-Nobusada and Hitoshi Sakakibara (2007) Over-expression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism. Plant Cell Physiology, 48: in press.
