|
| Status |
Public on Nov 21, 2006 |
| Title |
D_U133Plus_114_nAcc |
| Sample type |
RNA |
| |
|
| Source name |
Postmorten human brain: Nucleus Accumbens
|
| Organism |
Homo sapiens |
| Characteristics |
GeneChip_Assay_Site: D
Original_GeneChip_Type: U133Plus
Subject_ID: 114
Brain_Region: Nucleus Accumbens
Redo: No
Cohort: 4
Sample_Block: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133Plus Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
|
| Description |
Gene expression data from postmorten brain regions
|
| Data processing |
The data were analyzed with the GCRMA method using custom CDF file Hs133A_Hs_UG_3.
|
| |
|
| Submission date |
Nov 16, 2006 |
| Last update date |
Nov 20, 2006 |
| Contact name |
Fan Meng |
| E-mail(s) |
mengf@umich.edu
|
| Phone |
734-615-7099
|
| Organization name |
U of Michigan
|
| Department |
Psychiatry
|
| Lab |
Microarray
|
| Street address |
205 Zina Pitcher Place
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL4557 |
| Series (1) |
| GSE6306 |
Sample Matching by Inferred Agonal Stress in Gene Expression Analyses of the Brain |
|
