NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM144947 Query DataSets for GSM144947
Status Public on Nov 21, 2006
Title I_U133A_35_Amy
Sample type RNA
 
Source name Postmorten human brain: Amygdala
Organism Homo sapiens
Characteristics GeneChip_Assay_Site: I
Original_GeneChip_Type: U133A
Subject_ID: 35
Brain_Region: Amygdala
Redo: No
Cohort: 2
Sample_Block: 1
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Description Gene expression data from postmorten brain regions
Data processing The data were analyzed with the GCRMA method using custom CDF file Hs133A_Hs_UG_3.
 
Submission date Nov 16, 2006
Last update date Nov 20, 2006
Contact name Fan Meng
E-mail(s) mengf@umich.edu
Phone 734-615-7099
Organization name U of Michigan
Department Psychiatry
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platform ID GPL4557
Series (1)
GSE6306 Sample Matching by Inferred Agonal Stress in Gene Expression Analyses of the Brain

Data table header descriptions
ID_REF
VALUE GCRMA-calculated log2(Signal intensity) using custom CDF Hs133A_Hs_UG_3

Data table
ID_REF VALUE
AFFX-BioB-3_at 4.920785206
AFFX-BioB-5_at 5.607099233
AFFX-BioB-M_at 7.567756157
AFFX-BioC-3_at 7.959844686
AFFX-BioC-5_at 8.339503558
AFFX-BioDn-3_at 11.57050849
AFFX-BioDn-5_at 8.921113963
AFFX-CreX-3_at 13.58509888
AFFX-CreX-5_at 13.06348917
AFFX-DapX-3_at 2.356762056
AFFX-DapX-5_at 2.60232948
AFFX-DapX-M_at 2.524292075
AFFX-HSAC07/X00351_3_at 14.18297294
AFFX-HSAC07/X00351_5_at 11.65885635
AFFX-HSAC07/X00351_M_at 12.30821939
AFFX-hum_alu_at 15.24090452
AFFX-HUMGAPDH/M33197_3_at 14.08821222
AFFX-HUMGAPDH/M33197_5_at 12.4873483
AFFX-HUMGAPDH/M33197_M_at 13.26740264
AFFX-HUMISGF3A/M97935_3_at 6.805444826

Total number of rows: 12734

Table truncated, full table size 308 Kbytes.




Supplementary file Size Download File type/resource
GSM144947.CEL.gz 3.6 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap