|
| Status |
Public on Dec 31, 2014 |
| Title |
MD_Pr.13_mouse_SS_31102012_(Mouse430_2) |
| Sample type |
RNA |
| |
|
| Source name |
32D cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 32D
transduction: FLT3 ITD78
|
| Treatment protocol |
32D cells were stably transduced with the respective FLT3-ITD retroviral constructs. Infected cells (5x106) were starved for 3h followed by 1h TKI-treatment.
|
| Growth protocol |
32D cells were stably transduced with the respective FLT3-ITD retroviral constructs. Infected cells (5x106) were starved for 3h followed by 1h TKI-treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 32D cells transduced with the respective FLT3-ITD constructs using TRIzol (Life Technologies).
|
| Label |
Biotin
|
| Label protocol |
For each sample preparation, total RNA was converted into double-stranded cDNA by reverse transcription using the 3' IVT Express Kit from Affymetrix according to the manufacturer's recommendations.
|
| |
|
| Hybridization protocol |
Hybridization, washing, and staining protocols, respectively, were performed on Affymetrix GeneChip instruments (Hybridization Oven 640, Fluidics Station FS450) as recommended by the manufacturer.
|
| Scan protocol |
Scanning was performed on Affymetrix GeneChip Scanner GCS3000 instruments as recommended by the manufacturer using default settings. The software used was GCOS 1.2 or higher.
|
| Description |
Gene expression data from murine cells
|
| Data processing |
The data were analyzed with BRB ArrayTools using RMA. Intensity values were log2 transformed
|
| |
|
| Submission date |
Mar 26, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Lars Bullinger |
| E-mail(s) |
lars.bullinger@charite.de
|
| Phone |
+49-30-450-553111
|
| Organization name |
Charité
|
| Department |
Hematology, Oncology and Tumorimmunology
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE56241 |
Distinct biological and transcriptional features of FLT3-ITD variants in vitro and in vivo |
|
