|
Status |
Public on Sep 08, 2006 |
Title |
PAO1_PCP_6.5hr |
Sample type |
RNA |
|
|
Source name |
Whole cells
|
Organism |
Pseudomonas aeruginosa |
Characteristics |
Strain PAO1
|
Biomaterial provider |
E.P. Greenberg, University of Washington
|
Treatment protocol |
Treated with RNA Bacterial Protect Reagent (Qiagen, Valencia, CA)
|
Growth protocol |
Cells were grown in a chemostat at a 12 hour generation time in a minimal medium that contained acetate as the sole carbon source.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Kit from Qiagen, with on-column DNase-digestion as described by the manufacturer.
|
Label |
Biotin
|
Label protocol |
As described by Affymetrix
|
|
|
Hybridization protocol |
As described by Affymetrix
|
Scan protocol |
As described by Affymetrix
|
Description |
Second of four timepoints in experiment, pentachlorophenol concentration 15 mg/L, replicate 1 of 2
|
Data processing |
GCOS 1.1.1, using default parameters and scaled to 100 average intensity over entire genechip
|
|
|
Submission date |
Aug 25, 2006 |
Last update date |
Aug 28, 2006 |
Contact name |
Nancy G. Love |
E-mail(s) |
nlove@vt.edu
|
Phone |
540-231-3980
|
Fax |
540-231-7916
|
Organization name |
Virginia Tech
|
Department |
Civil and Environmental Engineering
|
Lab |
CEE Environmental Bionanotechnology Lab
|
Street address |
418 Durham Hall
|
City |
Blacksburg |
State/province |
VA |
ZIP/Postal code |
24061 |
Country |
USA |
|
|
Platform ID |
GPL84 |
Series (1) |
|