|
| Status |
Public on Mar 05, 2014 |
| Title |
Wild_type_Arabidopsis_2wks_seedling_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type Arabidopsis thaliana 2wks seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: wt
age: 2wks
tissue: whole seedlings
ecotype: Columbia-0
|
| Treatment protocol |
none
|
| Growth protocol |
All plants were grown in MS (1% sucrose) agar medium with or without hygromycin in 16hr light / 8hr dark cycle in 23 Celsius degree.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plant RNeasy Mini kit (QIAGEN) was used to isolate total RNA.
|
| Label |
Cy3
|
| Label protocol |
1 mg of total RNA was used for labeling procedure using Qick Amp Labeling kit (One-color) (Agilent).
|
| |
|
| Hybridization protocol |
Hybridized using Gene Expression Hybridization kit (Agilent) and washed by Gene Expression wash pack (Agilent).
|
| Scan protocol |
Scanned on an Agilent G2505B scanner.
|
| Description |
Five T2 seedlings were grown in MS agar medium and harvested for RNA extraction
control_rep3
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1).
Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction. After that, signal of each probe was divided by median value of filter-passed probes.
|
| |
|
| Submission date |
Oct 23, 2013 |
| Last update date |
Mar 05, 2014 |
| Contact name |
Nobutaka Mitsuda |
| E-mail(s) |
nobutaka.mitsuda@aist.go.jp
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Bioproduction Research Institute
|
| Lab |
Gene Regulation Research Group
|
| Street address |
Central 6 Higashi 1-1-1
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7299 |
| Series (1) |
| GSE51595 |
MYB87 chimera repressor suppresses longitudinal organ growth with aberrant expressions of cell wall related genes in Arabidopsis thaliana |
|
