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Status
Public on Aug 10, 2006
Title
IMR386
Sample type
genomic
Channel 1 Source name
Sample_IMR386
Organism
Homo sapiens Characteristics
Sample_IMR386
Biomaterial provider
Sam Knight, UK
Extracted molecule
genomic DNA
Extraction protocol
Phenol-chloroform extraction
Label
cy3
Label protocol
PMID: 15918152
Channel 2 Source name
GM15724
Organism
Homo sapiens Characteristics
Lymphoblastoid cell line GM15724
Biomaterial provider
Coriell Depositoryhttp://locus.umdnj.edu/nigms/nigms_cgi/display.cgi?GM15724
Growth protocol
Grown as per Coriell recommendations
Extracted molecule
genomic DNA
Extraction protocol
Gentra Puregene DNA Isolation system
Label
cy5
Label protocol
PMID: 15918152
Hybridization protocol
PMID: 15918152
Scan protocol
Slides scanned with ArrayWorX scanner. Image analysis and normalization were performed using UCSF Spot and Sproc software (Jain AN, Tokuyasu TA, Snijders AM, Segraves R, Albertson DG, Pinkel D (2002) Fully automatic quantification of microarray image data. Genome Res 12:325-332)
Description
BACs for which only one of the triplicates printed on the array yielded data, or for which the standard deviation of log2 ratio for the triplicates was >0.2, were removed from final analysis.
Data processing
Default parameters within Spot software
Submission date
Jul 21, 2006
Last update date
Aug 09, 2006
Contact name
Andrew Sharp
E-mail(s)
asharp@u.washington.edu Phone
(+1) 206 616 5117
Fax
(+1) 206 685 7301
Organization name
University of Washington
Department
Genome Sciences
Lab
Eichler Lab, HSB K360
Street address
1705 NE Pacific St
City
Seattle
State/province
WA
ZIP/Postal code
98195
Country
USA
Platform ID
GPL4010 Series (1)
GSE5373 Discovery of previously unidentified genomic disorders from the duplication architecture of the human genome
Data table header descriptions ID_REF VALUE Normalized mean log2 ratio for triplicate spots of that clone on the array
Data table ID_REF VALUE HS7E10
0.076318
HS5F7
0.049595
HS2C10
-0.020392
HS15C8
HS21H10
-0.0235
HS13E4
0.012915
HS21D9
-0.068226
HS15E4
-0.104726
HS11F5
0.45287
HS21E9
0.118761
HS17E12
-0.003033
HS23D3
HS7G11
0.102201
HS13B4
0.058846
HS2G2
-0.004806
HS11C11
0.260995
HS2B7
0.079947
HS12D8
0.187418
HS13C7
-0.128554
HS5B8
-0.154305
2043 31 Kbytes .Supplementary file Size Download File type/resource GSM122692.txt.gz
781.4 Kb
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