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Sample GSM121994 Query DataSets for GSM121994
Status Public on Jul 23, 2008
Title Thyroid normal sample 3
Sample type RNA
 
Source name Thyroid normal sample
Organism Homo sapiens
Characteristics Tissue: thyroid
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 microg total RNA (Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
Description Gene expression data from thyroid cancer
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Jul 20, 2006
Last update date Jul 22, 2008
Contact name Kun Yu
E-mail(s) yukun@omniarray.com
Phone +65 64368390
Organization name National Cancer Centre
Department Cellular & Molecular Research
Street address
City Singapore
ZIP/Postal code 169610
Country Singapore
 
Platform ID GPL96
Series (1)
GSE5364 A Precisely Regulated Gene Expression Cassette Potently Modulates Metastasis and Survival in Multiple Solid Cancers

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call. P<0.04 is used to filter the intensity value of probe

Data table
ID_REF VALUE DETECTION P-VALUE
200594_x_at 6201.2627 2.44E-04
200595_s_at 1848.5658 2.44E-04
200596_s_at 751.8427 7.32E-04
200597_at 471.32513 0.001953125
200598_s_at 5346.57 2.44E-04
200599_s_at 10031.882 2.44E-04
200600_at 3046.1309 2.44E-04
200602_at 2183.2473 2.44E-04
200603_at 4821.8696 2.44E-04
200604_s_at 1424.2793 2.44E-04
200605_s_at 3569.6218 2.44E-04
200606_at 4669.019 2.44E-04
200607_s_at 647.7965 0.002929688
200608_s_at 1539.6343 2.44E-04
200609_s_at 3295.1497 2.44E-04
200610_s_at 5308.6597 2.44E-04
200611_s_at 3849.734 2.44E-04
200612_s_at 1230.6395 0.014160156
200613_at 1521.4873 0.001953125
200614_at 5448.526 2.44E-04

Total number of rows: 22283

Table truncated, full table size 682 Kbytes.




Supplementary data files not provided

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