|
| Status |
Public on Jul 23, 2008 |
| Title |
Breast tumor sample 22 |
| Sample type |
RNA |
| |
|
| Source name |
Breast tumor sample
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: breast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 microg total RNA (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
|
| Description |
Gene expression data from breast cancer
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jul 20, 2006 |
| Last update date |
Jul 22, 2008 |
| Contact name |
Kun Yu |
| E-mail(s) |
yukun@omniarray.com
|
| Phone |
+65 64368390
|
| Organization name |
National Cancer Centre
|
| Department |
Cellular & Molecular Research
|
| Street address |
|
| City |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE5364 |
A Precisely Regulated Gene Expression Cassette Potently Modulates Metastasis and Survival in Multiple Solid Cancers |
|
