gender: female age (yrs): 71 diagnosis: rheumatoid arthritis tissue: plasma
Treatment protocol
n/a
Growth protocol
n/a
Extracted molecule
total RNA
Extraction protocol
RT2 qPCR-Grade miRNA isolation kit (SABiosciences, Frederick, MD) after twice extration using with Sepasol-RNA II (Nacalai Tesque, Kyoto, Japan), a phenol-based reagent for liquid sample.
The SDS2.2.2 automatically caluculated the uploaded raw threshold cycle data. Normalization was carried out with the average Ct value of all miRNAs. Relative quantification of miRNA expression was calculated with the 2−ΔΔCt Ct method. The data was presented as log10 of the relative quantity of each miRNA. Fold Change worksheet reports test/control (i.e., RA/HC) ratios.
